Abstract A simple and rapid method for the simultaneous determination of 35 non-derivatized metabolites was developed using liquid chromatography – tandem mass spectrometry. Under the optimal conditions, linearity was achieved in the range from 0.05 to 10.0 ng (R2 ≥ 0.989). The repeatability provided percentage relative standard deviations ≤11.2 and a trueness expressed as percentage relative error from −10.7% to 12%. The recoveries ranged from 87.3% to 109.8% with good repeatability. When applied to mouse lung tissue, plasma, and urine samples, 30 amino acids, creatine, creatinine, and oxidized glutathione were positively identified and quantified. The described present method was useful for the simultaneous determination of amino acids, creatine, creatinine, and oxidized glutathione in biological samples, including tissue, plasma, and urine.

中文翻译：

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使用液相色谱-串联质谱法同时测定肺组织、血浆和尿液中 35 种有机代谢物，包括氨基酸、肌酸、肌酐和氧化还原型谷胱甘肽

摘要 开发了一种使用液相色谱-串联质谱法同时测定 35 种非衍生代谢物的简单快速方法。在最佳条件下，线性度在 0.05 至 10.0 ng (R2 ≥ 0.989) 范围内实现。可重复性提供的百分比相对标准偏差≤11.2，准确度表示为从 -10.7% 到 12% 的百分比相对误差。回收率范围为 87.3% 至 109.8%，具有良好的重复性。当应用于小鼠肺组织、血浆和尿液样本时，30 种氨基酸、肌酸、肌酐和氧化型谷胱甘肽被阳性鉴定和定量。所描述的本方法可用于同时测定生物样品（包括组织、血浆和尿液）中的氨基酸、肌酸、肌酐和氧化型谷胱甘肽。