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LPA5-mediated signaling induced by endothelial cells and anticancer drug regulates cellular functions of osteosarcoma cells.
Experimental Cell Research ( IF 3.7 ) Pub Date : 2020-01-02 , DOI: 10.1016/j.yexcr.2020.111813
Kanako Minami 1 , Nanami Ueda 1 , Kaichi Ishimoto 1 , Toshifumi Tsujiuchi 1
Affiliation  

Lysophosphatidic acid (LPA) signaling via LPA receptors (LPA1 to LPA6) regulates a variety of malignant properties of cancer cells. It is known that endothelial cells promote tumor progression and chemoresistance. The present study aimed to investigate the roles of LPA5 in cellular functions modulated by endothelial cells and anticancer drug in osteosarcoma cells. Human osteosarcoma MG-63 cells were maintained in endothelial F2 cell supernatants. After culturing for 3 months, MG63-F2 cells were established. LPAR5 expression level in MG63-F2 cells was significantly elevated, compared with MG-63 cells. The cell motile activity of MG63-F2 cells was markedly higher than that of MG-63 cells. To validate the effects of LPA5 on cell motile activity, LPA5 knockdown cells were generated from MG-63 cells. The cell motile activity of MG-63 cells was inhibited by LPA5 knockdown. The cell survival to cisplatin (CDDP) was reduced in MG-63 cells treated with LPA. In the presence of LPA, the cell survival rate was significantly lower in MG63-F2 cells than MG-63 cells, correlating with LPAR5 expression. LPA5 knockdown cells indicated the high cell survival rate to CDDP. Moreover, LPAR5 expression level was increased in the long-term CDDP treated MG63-C cells. The cell survival to CDDP of MG63-C cells was enhanced by LPA5 knockdown. These results suggest that cellular functions are regulated through LPA5-mediatd signaling induced by endothelial cells and CDDP in MG-63 cells.

中文翻译:

内皮细胞和抗癌药诱导的LPA5介导的信号传导调节骨肉瘤细胞的细胞功能。

通过LPA受体(LPA1至LPA6)的溶血磷脂酸(LPA)信号调节癌细胞的多种恶性特性。已知内皮细胞促进肿瘤进展和化学抗性。本研究旨在探讨LPA5在骨肉瘤细胞中由内皮细胞和抗癌药物调节的细胞功能中的作用。人骨肉瘤MG-63细胞保持在内皮F2细胞上清液中。培养3个月后,建立MG63-F2细胞。与MG-63细胞相比,MG63-F2细胞中的LPAR5表达水平显着升高。MG63-F2细胞的细胞运动活性显着高于MG-63细胞。为了验证LPA5对细胞运动活性的影响,从MG-63细胞产生了LPA5敲低的细胞。LPA5敲低抑制了MG-63细胞的细胞运动活性。用LPA处理的MG-63细胞的顺铂(CDDP)细胞存活率降低。在存在LPA的情况下,MG63-F2细胞的细胞存活率显着低于MG-63细胞,与LPAR5表达相关。LPA5敲低细胞表明对CDDP的高细胞存活率。此外,长期CDDP处理的MG63-C细胞中LPAR5表达水平增加。LPA5敲低可提高MG63-C细胞对CDDP的存活率。这些结果表明,通过MG-63细胞中的内皮细胞和CDDP诱导的LPA5介导的信号传导调节细胞功能。MG63-F2细胞的细胞存活率明显低于MG-63细胞,这与LPAR5的表达有关。LPA5敲低细胞表明对CDDP的高细胞存活率。此外,长期CDDP处理的MG63-C细胞中LPAR5表达水平增加。LPA5敲低可提高MG63-C细胞对CDDP的存活率。这些结果表明,通过MG-63细胞中的内皮细胞和CDDP诱导的LPA5介导的信号传导调节细胞功能。MG63-F2细胞的细胞存活率明显低于MG-63细胞,这与LPAR5的表达有关。LPA5敲低细胞表明对CDDP的高细胞存活率。此外,在长期CDDP处理的MG63-C细胞中,LPAR5表达水平增加。LPA5敲低可提高MG63-C细胞对CDDP的存活率。这些结果表明,通过MG-63细胞中的内皮细胞和CDDP诱导的LPA5介导的信号传导调节细胞功能。
更新日期:2020-01-02
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