This study aimed to explore the dynamic changes in metabolite profiles and metabolism pathways in the serum of growing pigs by intravenous infusion of sodium butyrate (SB). Fourteen crossbred growing barrows (BW = 23.70 ± 1.29 kg) fitted with jugular cannula were randomly allocated to the SB and control (Con) groups, each group consisted of seven replicates (pens), with one pig per pen. At 9:00 of each day during the experimental period, pigs in the SB group were infused with 10 mL of SB (200 mmol/L, pH 7.4, 37 °C) via precaval vein, while the Con group was treated with the same volume of physiological saline. On day 4, the blood of each pig was collected at 0, 30, 60, and 120 min after the intravenous infusion. Metabolites in the serum were detected by gas chromatograph-mass spectrometry analysis. Pathway analysis of metabolomic profiles showed that the differential metabolites mainly enriched in amino acid metabolism, lipid-related metabolism, and the tricarboxylic acid (TCA) cycle. More importantly, the relative concentrations of all eight essential amino acids, five non-essential amino acids, and two amino acid derivatives were decreased by the parenteral SB. In addition, SB significantly increased the relative concentrations of eicosanoic acid and octadecanoic acid and decreased the relative concentration of glycerol-3-phosphate at 0 min (three days after intravenous infusion of SB), which suggests that parenteral SB may increase stearates mobilization and decrease the biosynthesis of stearates. In conclusion, intravenous infusion of SB may induce more amino acids to synthesize proteins and affect fat metabolism through increasing fat mobilization and decreasing the biosynthesis of stearates. However, a further study is needed to understand the mechanism of extensive metabolic pathway changes induced by parenteral SB.

中文翻译：

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静脉注射丁酸钠诱导生长中猪血清代谢组学特征的动态变化。

这项研究旨在探讨通过静脉内输注丁酸钠（SB）来生长的猪血清中代谢物谱和代谢途径的动态变化。将14头装有颈静脉插管的杂种生长公猪（BW = 23.70±1.29 kg）随机分配给SB和对照组（Con）组，每组由7个重复（围栏）组成，每只猪圈养一头猪。在实验期间的每天9：00，SB大鼠通过腔静脉给其注入10 mL SB（200 mmol / L，pH 7.4，37°C），而Con组则用相同的剂量处理。生理盐水的体积。在第4天，在静脉输注后0、30、60和120分钟收集每只猪的血液。通过气相色谱-质谱分析法检测血清中的代谢物。代谢组学特征的通路分析表明，差异代谢物主要富集氨基酸代谢，脂质相关代谢和三羧酸（TCA）循环。更重要的是，肠胃外SB降低了全部八个必需氨基酸，五个非必需氨基酸和两个氨基酸衍生物的相对浓度。此外，SB在0分钟（静脉注射SB后3天）时，显着增加了二十碳酸和十八烷酸的相对浓度，并降低了3-磷酸甘油的相对浓度，这表明肠胃外SB可能会增加硬脂酸盐的动员和降低。硬脂酸盐的生物合成。综上所述，静脉内注射SB可能会诱导更多氨基酸合成蛋白质，并通过增加脂肪动员和减少硬脂酸酯的生物合成来影响脂肪代谢。但是，需要进一步的研究来了解肠胃外SB引起的广泛代谢途径改变的机制。