The effectiveness of novel polymeric nanoparticles (NPs) application in reducing dentin permeability and facilitating dentin remineralization after endodontic treatment was evaluated. The effect of undoped NPs, zinc, calcium and doxycycline-doped NPs (Zn-NPs, Ca-NPs and D-NPs, respectively) was tested in radicular dentin. A control group without NPs was included. Radicular dentin was assessed for fluid filtration. Dentin remineralization was analyzed by scanning and transmission electron microscopy, energy-dispersive analysis, AFM, Young's modulus (Ei), Nano DMA, Raman, and X-Ray Diffraction analysis. Ca-NPs and Zn-NPs treated dentin exhibited the lowest microleakage with hermetically sealed dentinal tubules and a zinc-based salt generation onto dentin. Zn-NPs favored crystallinity and promoted the highest Ei and functional remineralization at the apical dentin, generating differences between the values of complex modulus among groups. Ca-NPs produced closure of tubules and porosities at the expense of a relative mineral amorphization, without creating zones of stress concentration. The highest sealing efficacy was obtained in Zn-NPs-treated samples, along with the highest values of Young's modulus and dentin mineralization. These high values of Ei were obtained by closing voids, cracks, pores and tubules, and by strengthening the root dentin. When using undoped NPs or Ca-NPs, deposition of minerals occurred, but radicular dentin was not mechanically reinforced. Therefore, application of Zn-NPs in endodontically treated teeth previous to the canal filling is encouraged.

评估了新型聚合物纳米颗粒（NPs）在降低牙本质通透性和促进牙髓治疗后牙本质再矿化方面的有效性。在放射状牙本质中测试了未掺杂的NP，锌，钙和强力霉素掺杂的NP（分别为Zn-NP，Ca-NP和D-NP）的作用。包括没有NP的对照组。评估了根部牙本质的液体过滤。通过扫描和透射电子显微镜，能量色散分析，原子力显微镜，杨氏模量（E i），纳米DMA，拉曼和X射线衍射分析对牙本质再矿化进行了分析。Ca-NPs和Zn-NPs处理过的牙本质表现出最低的微渗漏和密闭的牙本质小管，并且在牙本质上生成锌基盐。Zn-NPs有利于结晶并促进最高顶端牙本质的Ei和功能性再矿化，在各组之间的复数模量值之间产生差异。Ca-NPs造成小管和孔隙的封闭，但以相对的矿物非晶化为代价，而没有产生应力集中区域。在Zn-NPs处理过的样品中获得了最高的密封效果，同时杨氏模量和牙本质矿化的值也最高。这些高Ei值是通过封闭空隙，裂缝，孔洞和小管并通过增强牙本质牙本质来获得的。当使用未掺杂的NP或Ca-NP时，会发生矿物质沉积，但放射状牙本质没有得到机械增强。因此，鼓励在牙根管充填之前在经牙髓治疗的牙齿中使用Zn-NPs。