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HPLC-ICP-MS speciation of selenium in Se-cultivated Flammulina velutipes
Arabian Journal of Chemistry ( IF 6 ) Pub Date : 2020-01-01 , DOI: 10.1016/j.arabjc.2017.05.012
Mei Tie , Baorui Li , Tiebiao Sun , Wei Guan , Yanqiu Liang , Huawei Li

Abstract Selenium is an essential micronutrient required at trace levels for human health, and dietary intake is the only source of selenium, which appears mainly in the form of selenocompounds. In this study, Flammulina velutipes was grown for 80 days in standard medium containing selenite, and the level of total selenium in the organism was then determined by inductively-coupled plasma mass spectrometry (ICP-MS). In Se-cultivated F. velutipes, selenium was mainly distributed in the water-soluble form and the content of soluble selenium-containing species in Se-cultivated F. velutipes was 47.10 mg kg−1, accounted for 72.5% of the total selenium content. The water-soluble proteins in F. velutipes were extracted and precipitated by different ammonium sulfate saturation concentrations. Size-exclusion high performance liquid chromatography (SEC-HPLC) analysis of these proteins revealed the presence of at least six selenium-containing protein species, with molecular weights ranging from 9000 to 74,000 Da, Selenium-containing proteins represented about 7.0% of the total soluble selenium. The result of this study suggested that Se-cultivated F. velutipes could potentially be considered as a selenium supplement for human.

中文翻译:

硒培养金针菇中硒的 HPLC-ICP-MS 形态分析

摘要 硒是人体健康必需的微量微量元素,膳食摄入是硒的唯一来源,硒主要以硒化合物的形式存在。在这项研究中,金针菇在含有亚硒酸盐的标准培养基中生长 80 天,然后通过电感耦合等离子体质谱法 (ICP-MS) 测定生物体中的总硒水平。在Se栽培的F. velutipes中,硒主要以水溶性形式分布,Se栽培的F. velutipes中可溶性含硒物种的含量为47.10 mg kg-1,占总硒含量的72.5% . F. velutipes 中的水溶性蛋白质通过不同的硫酸铵饱和浓度进行提取和沉淀。对这些蛋白质的体积排阻高效液相色谱 (SEC-HPLC) 分析表明,至少存在 6 种含硒蛋白质,分子量范围为 9000 至 74,000 Da,含硒蛋白质约占总量的 7.0%可溶性硒。这项研究的结果表明,硒培养的 F. velutipes 可能被认为是人类的硒补充剂。
更新日期:2020-01-01
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