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Molecular characterization, expression and enzyme activity of three glutathione S-transferase genes from Eriocheir sinensis under pesticide stresses.
Comparative Biochemistry and Physiology C: Toxicology & Pharmacology ( IF 3.9 ) Pub Date : 2019-12-31 , DOI: 10.1016/j.cbpc.2019.108700
Xiao Zhang 1 , Guoqing Shen 1 , Yang Wang 1 , Pengdan Huang 1 , Kassimu Hashim Ame 1 , Yanan Zang 1 , Huaishun Shen 1
Affiliation  

Glutathione S-transferases (GSTs) are a multifunctional protein superfamily that can catalyze the detoxification processes in an organism. In the present study, we determined the structure and function of GSTs in Chinese mitten crab (Eriocheir sinensis) by gene cloning, expression, and enzyme activity in order to investigate the metabolic detoxification of GSTs in the hepatopancreas and muscles under three pesticide (trichlorfon, β-cypermethrin and avermectin) stresses. Multiple sequence alignment analysis showed that all the three Es-GST genes possessed N-terminal, and C-terminal domain as well as G-binding sites, while Es-GST2 and Es-GST3 contained Mu-type GST-specific Mu-loop structures. Phylogenetic tree analysis revealed that the three Es-GSTs belonged to the Mu-type GST of crustaceans. The quantitative real-time PCR revealed that the three Es-GSTS were expressed in 9 tissues of Eriocheir sinensis, with highest expression in hepatopancreas and muscle. The expression of the three Es-GSTS significantly increased in the hepatopancreas and muscle under the three pesticide stresses compared to the control group, and a steady increase in GST activity was observed. The study showed that the three Es-GSTs belong to the Mu-type GST of the crustaceans and might play an important role in the metabolic detoxification in Eriocheir sinensis.

中文翻译:

农药胁迫下中华绒螯蟹三个谷胱甘肽S-转移酶基因的分子表征,表达和酶活性。

谷胱甘肽S-转移酶(GST)是一种多功能蛋白质超家族,可以催化生物体中的排毒过程。在本研究中,我们通过基因克隆,表达和酶活性确定中华绒螯蟹(中华绒螯蟹)中GST的结构和功能,以研究在三种农药(三氯敌草, β-氯氰菊酯和阿维菌素)引起的压力。多重序列比对分析显示,所有三个Es-GST基因均具有N末端和C末端结构域以及G结合位点,而Es-GST2和Es-GST3包含Mu型GST特异性Mu环结构。系统进化树分析表明,这三种Es-GSTs属于甲壳类的Mu型GST。实时荧光定量PCR结果显示,三个Es-GSTS在中华绒螯蟹的9个组织中均有表达,在肝胰腺和肌肉中的表达最高。与对照组相比,在三种农药胁迫下,三种胰腺癌细胞和肌肉中三种Es-GSTS的表达均显着增加,并且观察到GST活性稳定增加。研究表明,三种Es-GST属于甲壳类动物的Mu型GST,可能在中华绒螯蟹的代谢排毒中起重要作用。并且观察到GST活性稳定增加。研究表明,三种Es-GST属于甲壳类动物的Mu型GST,可能在中华绒螯蟹的代谢排毒中起重要作用。并且观察到GST活性稳定增加。研究表明,三种Es-GST属于甲壳类动物的Mu型GST,可能在中华绒螯蟹的代谢排毒中起重要作用。
更新日期:2019-12-31
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