Antrodia camphorata is a rare and valuable medicinal mushroom. In this work, 11 new triterpenoids, namely, antcamphorols A-K (1-11), together with 10 known triterpenoids, 12-21, were isolated from dish-cultured A. camphorata. Compound 1 is an unprecedented C31 lanostane-type triterpenoid featuring a methyl group at C-15 and a C-21-O-C-24 tetrahydropyran ring at C-17. Compounds 2-11 are ergostane-type triterpenoids, and they include two pairs of norergostanes 2-5. The structures of the new compounds were identified by NMR, 2D NMR, and HRESIMS data analyses. The absolute configurations of 1 and 6 were defined by X-ray diffraction data, and the absolute configuration at C-25 of 4 was determined by the modified Mosher's method. Compounds 7, 9, 10, 16, and 19 showed significant ROS scavenging activities (63.9-70.5% at 20 μM) in high-glucose-induced HUVECs. Compounds 3 and 8 exhibited moderate cytotoxic activities against U251 (IC50, 9.2 μM) and MCF-7 (IC50, 8.1 μM) human cancer cell lines, respectively.

中文翻译：

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樟芝樟脑中的樟脑酚AK，细胞毒性和ROS清除三萜类化合物。

樟芝牛樟芝是一种稀有且有价值的药用蘑菇。在这项工作中，从菜培养的樟脑樟脑中分离出11种新的三萜类化合物，即antcamphorols AK（1-11），以及10种已知的三萜类化合物12-21。化合物1是前所未有的C31羊毛甾烷型三萜，在C-15处具有甲基，在C-17处具有C-21-OC-24四氢吡喃环。化合物2-11是麦角甾烷型三萜，并且它们包括两对降冰片烷烃2-5。通过NMR，2D NMR和HRESIMS数据分析鉴定了新化合物的结构。1和6的绝对构型由X射线衍射数据确定，C-25处4的绝对构型由改进的Mosher方法确定。化合物7、9、10、16和19表现出显着的ROS清除活性（63.9-70。在高葡萄糖诱导的HUVEC中为20μM时为5％）。化合物3和8分别显示出对U251（IC50，9.2μM）和MCF-7（IC50，8.1μM）人癌细胞系的中等细胞毒活性。