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Assessment of Phytochemical Compounds in Functional Couscous: Determination of Free and Bound Phenols and Alkylresorcinols
Food Research International ( IF 8.1 ) Pub Date : 2019-12-31 , DOI: 10.1016/j.foodres.2019.108970
Manuela Oriente , Elixabet Díaz-de-Cerio , Vito Verardo , Maria Cristina Messia , Ana María Gómez-Caravaca , Emanuele Marconi

The aim of this study was to evaluate the content of free and bound phenols and alkylresorcinols in functional couscous enriched with barley coarse fraction obtained by air classification. Two different levels of enriched barley flour (20 and 30%) were used for the formulation of couscous and they were compared with a control couscous made with 100% of semolina. HPLC-ESI-TOF-MS was used to determine the phenolic composition in couscous samples. Total free phenolic compounds content in control couscous was 182 μg/100g d.m.. This amount increases significantly when coarse barley flour is added reaching value of 2273 μg/100g d.m. and 2978 μg/100g d.m. when 20 and 30 % of barley coarse fraction was used, respectively. Moreover, the main free phenolic compounds in enriched barley samples are represented by flavan-3-ols. Bound phenols ranged from 5242 μg/100g d.m. for control couscous to 27092 μg/100g d.m. for couscous with 30% of barley coarse fraction with a strong prevalence of phenolic acids. Finally, regarding alkylresorcinol compounds, they ranged from 1.01 mg/g d.m. for control couscous to 2.46 mg/g d.m. for couscous with 30% of barley coarse fraction. In conclusion, on the basis of obtained results, barley coarse fraction can be considered a good ingredient to develop functional couscous naturally enriched of phenolic compounds and alkylresorcinols.



中文翻译:

功能性蒸粗麦粉中植物化学化合物的评估:游离和结合的苯酚和烷基间苯二酚的测定

这项研究的目的是评估通过空气分类获得的富含大麦粗粒的功能性蒸粗麦粉中游离和结合的酚和烷基间苯二酚的含量。两种不同水平的浓缩大麦粉(20%和30%)用于蒸粗麦粉的配制,并与100%粗面粉制成的对照蒸粗麦粉进行了比较。HPLC-ESI-TOF-MS用于测定蒸粗麦粉样品中的酚类成分。对照蒸粗麦粉中的总游离酚类化合物含量为182μg/ 100g dm。当添加粗大麦粉时,当分别使用20%和30%的大麦粗粒时,该值显着增加,达到2273μg/ 100g dm和2978μg/ 100g dm。此外,富集大麦样品中的主要游离酚类化合物以黄烷-3-醇为代表。结合酚的含量范围从对照蒸粗麦粉的5242μg/ 100g dm到蒸粗麦粉的27092μg/ 100g dm,其中大麦粗粒含量为30%,酚酸含量很高。最后,关于烷基间苯二酚化合物,其浓度范围从对照蒸粗麦粉的1.01 mg / g dm到蒸粗麦粉的2.46 mg / g dm,其中大麦粗粒含量为30%。总之,根据获得的结果,大麦粗馏分可被视为开发天然富含酚类化合物和烷基间苯二酚的功能蒸粗麦粉的良好成分。

更新日期:2019-12-31
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