Camelina sativa L. Crantz (large‐seeded false flax) is a promising oilseed crop for the production of edible oil and biodiesel. An ethyl methanesulfonate (EMS) mutant of C. sativa was identified that lacked seed coat mucilage (SCM) using Ruthenium Red (RR) colorimetric staining. Compared with wild‐type (WT) plants, the mucilage‐defect mutant line (Cs98) had smaller seeds and seeds with significantly less SCM, but exhibited significantly taller plant height. The seed mass and oil content of the seeds of Cs98 were significantly lower than those of WT plants. However, the seeds of Cs98 had significantly higher crude protein and starch contents, but a significantly lower neutral detergent‐soluble fiber (NDSF) fraction (pectin) content. Although Cs98 seed contained significantly higher mineral contents for various macro‐ and microminerals (e.g., Mg, S, Al, Cu, Mn, Fe, and Zn), these large differences did not prevent the Cs98 seed biodiesel from passing all American Society for Testing and Materials (ASTM) standards for macro‐ and micromineral content and viscosity, pH, and turbidity. Notably, the oil and biodiesel derived from Cs98 had significantly reduced viscosity compared with WT. Water washes of oil derived from WT and Cs98 seed confirmed that the Cs98 contained only 57% of the mucilage content of the WT oil washes. These significantly lower pectic residues are expected to improve the flow characteristics of the resultant oil and require less washing during biodiesel production.

中文翻译：

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降低了种子衣壳黏附度的茶树甲烷甲磺酸乙酯突变体的种子，油和脂肪酸甲酯的表征

亚麻茶（Camelina sativa L. Crantz）（大种子假亚麻）是一种有前途的油料作物，可用于生产食用油和生物柴油。苜蓿中华根瘤菌的甲磺酸乙酯（EMS）突变体使用钌红（RR）比色染色鉴定出缺少种皮粘液（SCM）。与野生型（WT）植物相比，粘液缺陷突变株（Cs98）的种子更小，种子的SCM明显更少，但株高却更高。Cs98种子的种子质量和含油量明显低于野生型植物。但是，Cs98的种子的粗蛋白和淀粉含量明显较高，但中性洗涤剂可溶性纤维（NDSF）分数（果胶）的含量却明显较低。尽管Cs98种子在各种宏观和微观矿物（例如Mg，S，Al，Cu，Mn，Fe和Zn）中所含矿物质含量都明显较高，这些巨大的差异并没有阻止Cs98种子生物柴油通过美国宏观材料和微观矿物含量以及粘度，pH和浊度的所有美国材料试验协会（ASTM）标准。值得注意的是，与WT相比，源自Cs98的油和生物柴油的粘度显着降低。来自WT和Cs98种子的油的水洗证实，Cs98仅包含WT油洗粘液含量的57％。这些明显较低的果胶残留物有望改善所得油的流动特性，并在生物柴油生产过程中需要较少的洗涤。来自WT和Cs98种子的油的水洗证实，Cs98仅包含WT油洗粘液含量的57％。这些明显较低的果胶残留物有望改善所得油的流动特性，并在生物柴油生产过程中需要较少的洗涤。来自WT和Cs98种子的油的水洗证实，Cs98仅包含WT油洗粘液含量的57％。这些明显较低的果胶残留物有望改善所得油的流动特性，并在生物柴油生产过程中需要较少的洗涤。