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The electrospun poly(ε‐caprolactone)/fluoridated hydroxyapatite nanocomposite for bone tissue engineering
Polymers for Advanced Technologies ( IF 3.4 ) Pub Date : 2019-12-30 , DOI: 10.1002/pat.4836
Narges Johari 1 , Mohammadhossein Fathi 2, 3 , Zeinab Fereshteh 4 , Saeid Kargozar 5 , Ali Samadikuchaksaraei 6, 7, 8
Affiliation  

Biodegradable cell‐incorporated scaffolds can guide the regeneration process of bone defects such as physiological resorption, tooth loss, and trauma which medically, socially, and economically hurt patients. Here, 0, 5, 10, and 15 wt% fluoridated hydroxyapatite (FHA) nanoparticles containing 25 wt% F and 75 wt% OH were incorporated into poly(ε‐caprolactone) (PCL) matrix to produce PCL/FHA nanocomposite scaffolds using electrospinning method. Then, scanning electron microscopy (SEM), X‐ray diffraction (XRD) pattern, and Fourier transform infrared spectroscopy (FTIR) were used to evaluate the morphology, phase structure, and functional groups of prepared electrospun scaffolds, respectively. Furthermore, the tensile strength and elastic modulus of electrospun scaffolds were investigated using the tensile test. Moreover, the biodegradation behavior of electrospun PCL/FHA scaffolds was studied by the evaluation of weight loss of mats and the alternation of pH in phosphate buffer saline (PBS) up to 30 days of incubation. Then, the biocompatibility of prepared mats was investigated by culturing MG‐63 osteoblast cell line and performing MTT assay. In addition, the adhesion of osteoblast cells on prepared electrospun scaffolds was studied using their SEM images. Results revealed that the fiber diameter of prepared electrospun PCL/FHA scaffolds alters between 700 and 900 nm. The mechanical assay illustrated the mat with 10 wt% FHA nanoparticles revealed the highest tensile strength and elastic modulus. The weight loss alternation of mats determined around 1% to 8% after 30 days of incubation. The biocompatibility and cell adhesion of mats improved by increasing the amounts of FHA nanoparticles.

中文翻译:

用于骨组织工程的电纺聚(ε-己内酯)/氟化羟基磷灰石纳米复合材料

可生物降解的细胞结合支架可以指导骨骼缺陷的再生过程,例如生理吸收,牙齿脱落和在医学,社会和经济上伤害患者的创伤。这里,0,5,10,和15%(重量)的羟基磷灰石氟化含有25重量%的F(FHA)的纳米颗粒-和75%(重量)OH -使用静电纺丝法将其掺入聚(ε-己内酯)(PCL)基质中以生产PCL / FHA纳米复合材料支架。然后,使用扫描电子显微镜(SEM),X射线衍射(XRD)图和傅立叶变换红外光谱(FTIR)分别评估了制备的电纺支架的形态,相结构和官能团。此外,使用拉伸试验研究了电纺支架的拉伸强度和弹性模量。此外,通过评估垫的失重和孵育长达30天的磷酸盐缓冲盐水(PBS)中pH的变化,研究了电纺PCL / FHA支架的生物降解行为。然后,通过培养MG-63成骨细胞系并进行MTT分析,研究了制备的席子的生物相容性。此外,使用SEM图像研究成骨细胞在制备的电纺丝支架上的粘附力。结果表明,制备的电纺PCL / FHA支架的纤维直径在700至900 nm之间变化。力学分析表明,含有10 wt%FHA纳米颗粒的垫子具有最高的拉伸强度和弹性模量。孵育30天后,垫子的重量损失交替约为1%至8%。垫子的生物相容性和细胞粘附性通过增加FHA纳米颗粒的量而得到改善。力学分析表明,含有10 wt%FHA纳米颗粒的垫子具有最高的拉伸强度和弹性模量。孵育30天后,垫子的重量损失交替约为1%至8%。垫子的生物相容性和细胞粘附性通过增加FHA纳米颗粒的量而得到改善。力学分析表明,含有10 wt%FHA纳米颗粒的垫子具有最高的拉伸强度和弹性模量。孵育30天后,垫子的重量损失交替约为1%至8%。垫子的生物相容性和细胞粘附性通过增加FHA纳米颗粒的量而得到改善。
更新日期:2019-12-30
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