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Leukocyte telomere length and serum polyunsaturated fatty acids, dietary habits, cardiovascular risk factors and features of myocardial infarction in elderly patients.
BMC Geriatrics ( IF 4.1 ) Pub Date : 2019-12-27 , DOI: 10.1186/s12877-019-1383-9
Are A Kalstad 1, 2 , Sjur Tveit 2, 3 , Peder L Myhre 3 , Kristian Laake 1 , Trine B Opstad 1, 2 , Arnljot Tveit 2, 4 , Erik B Schmidt 5 , Svein Solheim 1 , Harald Arnesen 1, 2 , Ingebjørg Seljeflot 1, 2
Affiliation  

BACKGROUND Telomeres are non-coding sequences at the end of eukaryote chromosomes, which in complex with associated proteins serve to protect subtelomeric DNA. Telomeres shorten with each cell division, are regarded as a biomarker for aging and have also been suggested to play a role in atherosclerosis and cardiovascular disease (CVD). The aim of the present study was to explore the associations between leukocyte telomere length and serum polyunsaturated fatty acids, diet, cardiovascular risk factors and features of myocardial infarction (MI) in elderly patients. METHODS The material is based upon the first 299 included patients in the OMEMI trial, where patients aged 70-82 years of age are randomized to receive omega-3 supplements or corn oil (placebo) after MI. Patients were included 2-8 weeks after the index MI. DNA was extracted from whole blood, and leukocyte telomere length (LTL) was analyzed by qPCR and reported as a number relative to a reference gene. Serum long chain polyunsaturated fatty acid (LCPUFA) content was analyzed by gas chromatography. Diet was evaluated with the validated SmartDiet food frequency questionnaire. Medical records, patient interviews and clinical examination provided previous medical history and anthropometric data. Non-parametric statistical tests were used. RESULTS Median (25, 75 percentile) LTL was 0.55 (0.42, 0.72). Patients had a median age of 75 years, 70.2% were male and 45.2% used omega-3 supplements. There was a weak, but significant correlation between LTL and linoleic acid (r = 0.139, p = 0.017), but not with other LCPUFAs. There was a trend towards longer telomeres with a healthier diet, but this did not reach statistical significance (p = 0.073). No associations were found between LTL and CVD risk factors or features of MI. CONCLUSIONS In our population of elderly with a recent myocardial infarction LTL was associated with linoleic acid concentrations, but not with other LCPUFAs. Patients with a healthy diet tended to have longer telomeres. The limited associations may be due to age and the narrow age-span in our population. Further studies, designed to detect longitudinal changes should be performed to explore the role of telomeres in cardiovascular aging. TRIAL REGISTRATION Clinical trials no. NCT01841944, registration date April 29, 2013.

中文翻译:

老年患者的白细胞端粒长度和血清多不饱和脂肪酸,饮食习惯,心血管危险因素以及心肌梗死的特征。

背景技术端粒是在真核生物染色体末端的非编码序列,其与相关蛋白复合用于保护亚端粒DNA。端粒随着每个细胞分裂而缩短,被认为是衰老的生物标记,并且还被认为在动脉粥样硬化和心血管疾病(CVD)中起作用。本研究的目的是探讨老年患者白细胞端粒长度与血清多不饱和脂肪酸,饮食,心血管危险因素和心肌梗死(MI)特征之间的关系。方法该材料基于OMEMI试验中前299名患者,其中70-82岁的患者在MI后被随机分配接受omega-3补充剂或玉米油(安慰剂)。指数MI后2-8周入组患者。从全血中提取DNA,并通过qPCR分析白细胞端粒长度(LTL),并报告为相对于参考基因的数字。血清长链多不饱和脂肪酸(LCPUFA)的含量通过气相色谱分析。使用经过验证的SmartDiet食物频率问卷对饮食进行评估。病历,患者访谈和临床检查提供了以前的病史和人体测量学数据。使用非参数统计检验。结果LTL的中位数(25,75%)为0.55(0.42,0.72)。患者的中位年龄为75岁,其中70.2%是男性,45.2%使用了omega-3补充剂。LTL和亚油酸之间存在弱但显着的相关性(r = 0.139,p = 0.017),而与其他LCPUFA之间没有相关性。饮食趋于健康的趋势是端粒更长,但这没有达到统计学显着性(p = 0.073)。在LTL和CVD危险因素或MI特征之间未发现关联。结论在我们最近患有心肌梗塞的老年人群中,LTL与亚油酸浓度有关,而与其他LCPUFA无关。饮食健康的患者倾向于端粒更长。关联性有限可能是由于年龄和我们人口中年龄段狭窄所致。应该进行旨在检测纵向变化的进一步研究,以探索端粒在心血管衰老中的作用。试验注册临床试验编号。NCT01841944,注册日期为2013年4月29日。结论在我们最近患有心肌梗塞的老年人群中,LTL与亚油酸浓度有关,而与其他LCPUFA无关。饮食健康的患者倾向于端粒更长。关联性有限可能是由于年龄和我们人口中年龄段狭窄所致。应该进行旨在检测纵向变化的进一步研究,以探索端粒在心血管衰老中的作用。试验注册临床试验编号。NCT01841944,注册日期为2013年4月29日。结论在我们最近患有心肌梗塞的老年人群中,LTL与亚油酸浓度有关,而与其他LCPUFA无关。饮食健康的患者倾向于端粒更长。关联性有限可能是由于年龄和我们人口中年龄段狭窄所致。应该进行旨在检测纵向变化的进一步研究,以探索端粒在心血管衰老中的作用。试验注册临床试验编号。NCT01841944,注册日期为2013年4月29日。应该进行旨在检测纵向变化的设计,以探索端粒在心血管衰老中的作用。试验注册临床试验编号。NCT01841944,注册日期为2013年4月29日。应该进行旨在检测纵向变化的设计,以探索端粒在心血管衰老中的作用。试验注册临床试验编号。NCT01841944,注册日期为2013年4月29日。
更新日期:2019-12-30
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