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Elevated expression of AGGF1 predicts poor prognosis and promotes the metastasis of colorectal cancer.
BMC Cancer ( IF 3.8 ) Pub Date : 2019-12-27 , DOI: 10.1186/s12885-019-6474-7
Xin Zhang 1 , Huimin Sun 2 , Wanyuan Chen 1 , Xianglei He 1
Affiliation  

BACKGROUND Angiogenic factor with G-patch and FHA domains 1 (AGGF1) can promote angiogenesis and increasing evidence has highlighted the important roles of AGGF1 in tumorigenesis. However, the differential expression as well as the biological functions of AGGF1 in colorectal cancer (CRC) remain to be established. The purpose of the present study is therefore to identify the effect of AGGF1 on prognosis and metastasis in CRC patients. METHODS The expression level of AGGF1 in CRC was examined by qPCR, western blot and immunohistochemistry in a tissue microarray containing 236 CRC specimens and paired normal mucosae. And the effect of AGGF1 on CRC cell malignance was investigated in our established stable AGGF1 upregulated and knockdown CRC cell lines. RESULTS The expression level of AGGF1 in CRC tissue was not significantly different to that in adjacent normal mucosa at the mRNA level. However, at the protein level, AGGF1 expression in CRC tissues was significantly higher than in paired normal mucosa, which showed a clear association with TNM stage, AJCC stage, vascular invasion, and differentiation. Further, we revealed an apparent correlation between AGGF1 expression and poorer disease-free survival and overall survival of CRC patients. In addition, we discovered that AGGF1 significantly promoted CRC cell wound healing, migration, and invasion in vitro and distant metastasis in vivo. CONCLUSIONS Our study demonstrates the aberrant overexpression of AGGF1 in CRC and provides a basis on which to explore the application of AGGF1 as a potential therapeutic target for CRC patients, especially for CRC patients with distant metastasis.

中文翻译:

AGGF1的表达升高预示着不良预后并促进了结直肠癌的转移。

背景技术具有G-膜片和FHA结构域1(AGGF1)的血管生成因子可以促进血管生成,越来越多的证据突出了AGGF1在肿瘤发生中的重要作用。但是,AGGF1在结直肠癌(CRC)中的差异表达和生物学功能仍有待建立。因此,本研究的目的是鉴定AGGF1对CRC患者的预后和转移的影响。方法采用qPCR,western blot和免疫组织化学方法检测236例CRC标本和配对正常黏膜的组织芯片中AGGF1的表达。并在我们建立的稳定的AGGF1上调和敲除的CRC细胞系中研究了AGGF1对CRC细胞恶性的影响。结果CRC组织中AGGF1的表达水平与癌旁正常黏膜的mRNA水平无明显差异。然而,在蛋白质水平上,CRC组织中AGGF1的表达明显高于配对的正常粘膜,这与TNM分期,AJCC分期,血管浸润和分化密切相关。此外,我们揭示了AGGF1表达与CRC患者的较差的无病生存率和总体生存率之间存在明显的相关性。此外,我们发现AGGF1在体外和体内远处转移中均显着促进CRC细胞伤口的愈合,迁移和侵袭。结论我们的研究证明了AGGF1在CRC中的异常过表达,并为探索AGGF1作为CRC患者潜在治疗靶点的应用提供了基础,
更新日期:2019-12-30
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