Light-activated phototherapy, including photothermal and photodynamic therapy, has become a new way for spatiotemporal control and noninvasive treatment of cancer. In this study, two new organic porphyrin molecules (NI-Por and NI-ZnPor) with donor (D)-acceptor (A) structure were designed and synthesized. The donor-acceptor pairs facilitated the intermolecular electron transfer, resulting in the enhancement of near-infrared (NIR) absorbance and nonradiative heat generation. After self-assembling, the nanoparticles were formed with the size around 60 nm. Relative to that of organic molecules, the absorption of NI-Por NPs and NI-ZnPor NPs broadened and red-shifted to the near-infrared region. Moreover, the porphyrin-containing nanoparticles can generate heat and reactive oxygen species (ROS) simultaneously induced by a single laser (635 nm). The intracellular reactive oxygen species production of NI-Por NPs and NI-ZnPor NPs was confirmed using DCFH-DA as an indicator. Furthermore, the localization of NI-Por NP and NI-ZnPor NP in HeLa cells was verified by fluorescence confocal laser microscopy. The photocytoxicity of two nanoparticles against HeLa cells was evaluated through the CCK-8 method. The IC50 of NI-Por NPs and NI-ZnPor NPs upon 635 nm laser irradiation was calculated to be 6.92 μg/mL and 5.86 μg/mL, respectively. Furthermore, the PDT/PTT synergistic effect of NPs under a 635 nm laser was verified through different treatment groups in vitro. All these results demonstrated that the as-prepared porphyrin-based nanoparticles are promising nanoagents for PDT/PTT in clinic.

中文翻译：

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具有DA结构的自组装萘二甲酰亚胺共轭卟啉纳米材料用于PDT / PTT协同治疗。

光活化光疗，包括光热疗法和光动力疗法，已经成为时空控制和癌症无创治疗的新途径。在这项研究中，设计并合成了两个具有供体（D）-受体（A）结构的新有机卟啉分子（NI-Por和NI-ZnPor）。供体-受体对促进了分子间电子的转移，从而提高了近红外（NIR）吸收率和非辐射热的产生。自组装后，形成尺寸为约60 nm的纳米颗粒。相对于有机分子，NI-Por NP和NI-ZnPor NP的吸收变宽并红移至近红外区域。此外，含卟啉的纳米粒子可产生热量和活性氧（ROS），而这些热量和活性氧是由单个激光（635 nm）同时诱导的。使用DCFH-DA作为指示剂，确认了NI-Por NPs和NI-ZnPor NPs的细胞内活性氧种类的产生。此外，通过荧光共聚焦激光显微镜验证了HeLa细胞中NI-Por NP和NI-ZnPor NP的定位。通过CCK-8方法评估了两种纳米粒子对HeLa细胞的光毒性。635 nm激光照射下NI-Por NP和NI-ZnPor NP的IC50分别计算为6.92μg/ mL和5.86μg/ mL。此外，通过不同的治疗组在体外验证了在635 nm激光下NPs的PDT / PTT协同作用。所有这些结果表明，所制备的基于卟啉的纳米粒子在临床上是用于PDT / PTT的有前途的纳米药物。