Urinary 1-hydroxypyrene (1-OHP), one of the metabolite of polycyclic aromatic hydrocarbons (PAHs), has been extensively applied as a biomarker of animals and human exposure to PAHs. Herein, we synthesized a novel conjugated polymer poly-8-methoxy-7-methyl-5-(10-anthracen-9-yl)quinolone (PMMAYQ) using Suzuki coupling reaction, and then fabricated a homogeneous PMMAYQ/grahene multilayer film via layer-by-layer self-assembly technique and applied for electrochemical sensing of 1-OHP. Under the optimal conditions, {PMMAYQ-Graphene}₁₆ modified glass carbon electrode exhibited excellent response for detection of 1-OHP. A linear range from 0.5 to 120 nM was obtained with a low detection limit of 0.07 nM (S/N = 3) and a sensitivity of 0.5539 μA/μM. The electrochemical sensor exhibited excellent stability, accepted repeatability, and good selectivity. The developed method was employed for determination of 1-OHP in human urine samples and recoveries in the range of 95.6%–107.1% were achieved.

尿素1-羟基py（1-OHP）是多环芳烃（PAHs）的代谢产物之一，已被广泛用作动物和人类暴露于PAHs的生物标志物。本文中，我们利用Suzuki偶联反应合成了新型共轭聚合物聚8-甲氧基-7-甲基-5-（10-蒽-9-基）喹诺酮（PMMAYQ），然后通过层法制备了均质的PMMAYQ /粒状多层膜层自组装技术并应用于1-OHP的电化学传感。在最佳条件下，{PMMAYQ-Graphene} ₁₆修饰的玻璃碳电极对1-OHP的检测显示出出色的响应。线性范围为0.5至120 nM，检测限为0.07 nM（S / N = 3），灵敏度为0.5539μA/μM。电化学传感器表现出优异的稳定性，可接受的重复性和良好的选择性。所开发的方法用于测定人类尿液样品中的1-OHP，回收率达到95.6％–107.1％。