Understanding the progression of periodontal tissue destruction is at the forefront of periodontal research. The authors aimed to capture the dynamics of gingival tissue proteome during the initiation and progression of experimental (ligature-induced) periodontitis in mice. Pressure cycling technology (PCT), a recently developed platform that uses ultra-high pressure to disrupt tissues, is utilized to achieve efficient and reproducible protein extraction from ultra-small amounts of gingival tissues in combination with liquid chromatography-tandem mass spectrometry (MS). The MS data are processed using Progenesis QI and the regulated proteins are subjected to METACORE, STRING, and WebGestalt for functional enrichment analysis. A total of 1614 proteins with ≥2 peptides are quantified with an estimated protein false discovery rate of 0.06%. Unsupervised clustering analysis shows that the gingival tissue protein abundance is mainly dependent on the periodontitis progression stage. Gene ontology enrichment analysis reveals an overrepresentation in innate immune regulation (e.g., neutrophil-mediated immunity and antimicrobial peptides), signal transduction (e.g., integrin signaling), and homeostasis processes (e.g., platelet activation and aggregation). In conclusion, a PCT-assisted label-free quantitative proteomics workflow that allowed cataloging the deepest gingival tissue proteome on a rapid timescale and provided novel mechanistic insights into host perturbation during periodontitis progression is applied.

中文翻译：

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牙周组织的压力循环技术辅助质谱定量显示了炎症性牙周疾病的发生和发展过程中的蛋白质组动力学。

了解牙周组织破坏的进展是牙周研究的重中之重。作者的目的是捕获小鼠实验性（结扎诱发的）牙周炎的开始和进展过程中牙龈组织蛋白质组的动力学。压力循环技术（PCT）是最近开发的使用超高压破坏组织的平台，结合液相色谱-串联质谱（MS）来实现从超少量牙龈组织中高效且可重现的蛋白质提取。 。使用Progenesis QI处理MS数据，并对调节的蛋白质进行METACORE，STRING和WebGestalt进行功能富集分析。总共对1614个具有≥2个肽段的蛋白质进行了定量，估计的蛋白质错误发现率为0.06％。无监督聚类分析表明，牙龈组织蛋白的丰度主要取决于牙周炎的进展阶段。基因本体论富集分析揭示了先天免疫调节（例如中性粒细胞介导的免疫和抗菌肽），信号转导（例如整联蛋白信号传导）和稳态过程（例如血小板活化和聚集）的过度表达。总之，应用了PCT辅助的无标签定量蛋白质组学工作流程，该流程允许在快速的时间范围内对最深的牙龈组织蛋白质组进行分类，并为牙周炎进展过程中的宿主摄动提供了新颖的机制。基因本体论富集分析揭示了先天免疫调节（例如中性粒细胞介导的免疫和抗菌肽），信号转导（例如整联蛋白信号传导）和稳态过程（例如血小板活化和聚集）的过度表达。总之，应用了PCT辅助的无标签定量蛋白质组学工作流程，该流程允许在快速的时间范围内对最深的牙龈组织蛋白质组进行分类，并为牙周炎进展过程中的宿主摄动提供了新颖的机制。基因本体论富集分析揭示了先天免疫调节（例如中性粒细胞介导的免疫和抗菌肽），信号转导（例如整联蛋白信号传导）和稳态过程（例如血小板活化和聚集）的过度表达。总之，应用了PCT辅助的无标签定量蛋白质组学工作流程，该流程允许在快速的时间范围内对最深的牙龈组织蛋白质组进行分类，并为牙周炎进展过程中的宿主摄动提供了新颖的机制。