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Extraction of sulfated agar from Gracilaria lemaneiformis using hydrogen peroxide-assisted enzymatic method.
Carbohydrate Polymers ( IF 11.2 ) Pub Date : 2019-12-28 , DOI: 10.1016/j.carbpol.2019.115790
Huijing Chen 1 , Qiong Xiao 2 , Huifen Weng 2 , Yonghui Zhang 3 , Qiuming Yang 1 , Anfeng Xiao 1
Affiliation  

In this study, an eco-friendly extraction method was explored to obtain high sulfate content agar and repair the deficiency of enzymatic extraction by taking full advantage of H2O2. The sulfate content of EHA (H2O2-assisted enzymatic extracted agar) reached 3.56 %, which is significantly higher than that of traditional alkali-extracted agar (AA, 1.8 %). Moreover, EHA exhibited lower viscosity (9.4 cP), which improved 26.6 % and 14 % of filtration and gel dehydration rates than EA (enzymatic extracted agar), respectively. Additionally, the physicochemical properties of the agars were evaluated and compared. Among these agars, EHA showed some favorable properties, such as high yield (16.08 %) and low dissolution temperature (88.9 °C). The surface of algae became smoother after treatment with H2O2 due to effective degradation of cellulose. Besides, mass spectrometry analysis revealed that EHA preserved a great amount of sulfate, while thermogravimetric analysis suggested that the thermal stability of EA and EHA both decreased.

中文翻译:

双氧水辅助酶法从江西Gra中提取硫酸琼脂。

在这项研究中,探索了一种生态友好的提取方法,以获得高硫酸盐含量的琼脂并通过充分利用H2O2来弥补酶法提取的不足。EHA(H2O2辅助酶促提取琼脂)的硫酸盐含量达到3.56%,显着高于传统的碱提取琼脂(AA,1.8%)。此外,EHA的粘度较低(9.4 cP),与EA(酶法提取的琼脂)相比,分别提高了26.6%和14%的过滤和凝胶脱水率。另外,评估和比较琼脂的物理化学性质。在这些琼脂中,EHA显示出一些有利的特性,例如高收率(16.08%)和低溶解温度(88.9°C)。由于纤维素的有效降解,用H2O2处理后,藻类表面变得更光滑。除了,
更新日期:2019-12-29
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