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Depicting the binding of furazolidone/furacilin with DNA by multiple spectroscopies, voltammetric as well as molecular docking.
Luminescence ( IF 2.9 ) Pub Date : 2019-12-28 , DOI: 10.1002/bio.3754
Shuyun Bi 1 , Xiaoyue Sun 1 , Xu Li 1 , Rui Zhao 1 , Di Shao 1
Affiliation  

The interaction between DNA and furazolidone/furacillin was investigated using various analytical techniques including spectroscopy and electroanalysis and molecular modelling. With the aid of acridine orange (AO), the fluorescence lifetimes of DNA-AO, DNA-furazolidone/furacillin-AO remained almost the same, which proved that the ground state complex was formed due to furazolidone/furacillin binding with DNA. Circular dichroism spectra and Fourier transform infrared spectroscopy showed that the second structure of DNA changed. Viscosity experiments presented that relative viscosity of DNA was increased with the increasing concentrations of furazolidone and almost unchanged for furacilin. In addition, the results of melting temperature (Tm ), ionic strength, site competition experiments, cyclic voltammetry, and molecular docking all proved the intercalation binding mode for furazolidone and groove binding mode for furacilin. The binding constants (Ka ) obtained from Wolfe-Shimmer equation were calculated as 3.66 × 104 L mol-1 and 3.95 × 104 L mol-1 for furazolidone-DNA and furacilin-DNA, respectively.

中文翻译:

通过多次分光光度法,伏安法以及分子对接来描述呋喃唑酮/呋喃西林与DNA的结合。

使用各种分析技术,包括光谱学,电分析和分子建模,研究了DNA与呋喃唑酮/呋喃西林之间的相互作用。借助于a啶橙(AO),DNA-AO,DNA-呋喃唑酮/呋喃西林-AO的荧光寿命几乎保持不变,这证明了由于呋喃唑酮/呋喃西林与DNA结合而形成了基态复合物。圆二色性光谱和傅里叶变换红外光谱表明,DNA的二级结构发生了变化。粘度实验表明,随着呋喃唑酮浓度的增加,DNA的相对粘度增加,而呋喃西林的DNA相对粘度几乎不变。此外,结果包括熔融温度(Tm),离子强度,现场竞争实验,循环伏安法,分子对接的结果证明了呋喃唑酮的嵌入结合方式和呋喃西林的沟槽结合方式。从Wolfe-Shimmer方程获得的结合常数(Ka)分别计算为呋喃唑酮-DNA和呋喃西林-DNA的3.66×104 L mol-1和3.95×104 L mol-1。
更新日期:2019-12-28
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