MicroRNAs (miRNAs) are a class of noncoding RNA molecules containing 18-24 nucleotides, and those with conserved structures are able to regulate the expression of eukaryotic genes by inhibition or enhancement of mRNA translation. However, miRNAs of the blue mussel, Mytilus galloprovincialis have not been reported. M. galloprovincialis is a primary species distributed along coastal zones worldwide. To reveal the repertoire of miRNAs in M. galloprovincialis, we constructed small RNA libraries prepared from three different mussels, which were then sequenced by Solexa deep sequencing technology. A total of 32,836,817, 33,359,113 and 33,093,562 clean reads from the tissues of the three M. galloprovincialis were obtained. Based on sequence similarities and hairpin structure predictions, 137 M. galloprovincialis miRNAs (mg-miRNA) were identified. Among the mg-miRNAs, 104 were conserved across species, whereas 33 might be novel and specific for M. galloprovincialis. Some of the mg-miRNAs, such as let-7 and the miR-100 family are playing key roles in many metabolic pathways and are worthy of further study. By performing a whole genome-scale characterization of mg-miRNAs and proposing their potential functions, these results provide a foundation for understanding the biological processes of the blue mussel, M. galloprovincialis.

中文翻译：

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海洋贻贝Mytilus galloprovincialis中的microRNA和mRNA的分析。

MicroRNA（miRNA）是一类包含18-24个核苷酸的非编码RNA分子，具有保守结构的MicroRNA能够通过抑制或增强mRNA翻译来调节真核基因的表达。但是，尚未报道蓝贻贝Mytilus galloprovincialis的miRNA。Galloprovincialis是一种分布在世界各地沿海地区的主要物种。为了揭示M. galloprovincialis中miRNA的组成，我们构建了由三种不同贻贝制备的小RNA文库，然后通过Solexa深度测序技术对其进行了测序。从三个鸡毒支原体的组织中获得了总计32,836,817、33,359,113和33,093,562的干净读数。根据序列相似性和发夹结构预测，鉴定了137个鸡pro.provocialis miRNA（mg-miRNA）。在mg-miRNA中，有104个在物种间保守，而33个可能是新颖的且对鸡传染性支原体具有特异性。一些mg-miRNA，例如let-7和miR-100家族在许多代谢途径中起着关键作用，值得进一步研究。通过对mg-miRNA进行全基因组规模的表征并提出其潜在功能，这些结果为理解蓝贻贝的生物学过程提供了基础。