Ustilago maydis is a promising yeast for the production of a range of valuable metabolites, including itaconate, malate, glycolipids and triacylglycerols. However, wild‐type strains generally produce a potpourri of all of these metabolites, which hinders efficient production of single target chemicals. In this study, the diverse by‐product spectrum of U. maydis was reduced through strain engineering using CRISPR/Cas9 and FLP/FRT, greatly increasing the metabolic flux into the targeted itaconate biosynthesis pathway. With this strategy, a marker‐free chassis strain could be engineered, which produces itaconate from glucose with significantly enhanced titre, rate and yield. The lack of by‐product formation not only benefited itaconate production, it also increases the efficiency of downstream processing improving cell handling and product purity.

中文翻译：

---

乌斯季亚戈氏菌可用于生产衣康酸而没有副产物的底盘。

玉米粉虱是一种有前途的酵母，可用于生产一系列有价值的代谢产物，包括衣康酸酯，苹果酸，糖脂和三酰基甘油。但是，野生型菌株通常会产生所有这些代谢产物的杂烩，这阻碍了单一目标化学品的有效生产。在这项研究中，五味U的多种副产物谱通过使用CRISPR / Cas9和FLP / FRT进行菌株工程可以减少糖原代谢，从而大大增加了进入目标衣康酸酯生物合成途径的代谢通量。通过这种策略，可以设计出无标记的底物菌株，该菌株可以从葡萄糖生产衣康酸酯，从而显着提高滴度，速率和产量。缺乏副产物的形成不仅有利于衣康酸酯的生产，而且还提高了下游加工的效率，从而改善了细胞处理能力和产品纯度。