Background The bioconversion of lignocellulose to fermentable C5/C6-saccharides is composed of pretreatment and enzymatic hydrolysis. Lignin, as one of the main components, resists lignocellulose to be bio-digested. Alkali and organosolv treatments were reported to be able to delignify feedstocks and loose lignocellulose structure. In addition, the use of additives was an alternative way to block lignin and reduce the binding of cellulases to lignin during hydrolysis. However, the relatively high cost of these additives limits their commercial application. Results This study explored the feasibility of using elephant grass (Pennisetum purpureum) and reed straw (Phragmites australis), both of which are important fibrous plants with high biomass, no-occupation of cultivated land, and soil phytoremediation, as feedstocks for bio-saccharification. Compared with typical agricultural residues, elephant grass and reed straw contained high contents of cellulose and hemicellulose. However, lignin droplets on the surface of elephant grass and the high lignin content in reed straw limited their hydrolysis performances. High hydrolysis yield was obtained for reed straw after organosolv and alkali pretreatments via increasing cellulose content and removing lignin. However, the hydrolysis of elephant grass was only enhanced by organosolv pretreatment. Further study showed that the addition of bovine serum albumin (BSA) or thioredoxin with His- and S-Tags (Trx-His-S) improved the hydrolysis of alkali-pretreated elephant grass. In particular, Trx-His-S was first used as an additive in lignocellulose saccharification. Its structural and catalytic properties were supposed to be beneficial for enzymatic hydrolysis. Conclusions Elephant grass and reed straw could be used as feedstocks for bioconversion. Organosolv and alkali pretreatments improved their enzymatic sugar production; however, the increase in hydrolysis yield of pretreated elephant grass was not as effective as that of reed straw. During the hydrolysis of alkali-pretreated elephant grass, Trx-His-S performed well as additive, and its structural and catalytic capability was beneficial for enzymatic hydrolysis.

中文翻译：

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通过添加硫氧还蛋白-His-S 的预处理和水解从大象草和芦苇秸秆中酶促糖生产。

背景 木质纤维素向可发酵 C5/C6 糖的生物转化由预处理和酶水解组成。木质素作为主要成分之一，可抵抗木质纤维素的生物消化。据报道，碱和有机溶剂处理能够使原料脱木质素和松散的木质纤维素结构。此外，添加剂的使用是阻断木质素并减少水解过程中纤维素酶与木质素结合的替代方法。然而，这些添加剂相对较高的成本限制了它们的商业应用。结果本研究探讨了利用象草（Pennisetum purpureum）和芦苇（Phragmites australis）这两种生物量高、不占用耕地、土壤植物修复的重要纤维植物作为生物糖化原料的可行性。 . 与典型的农残相比，象草和芦苇秸秆的纤维素和半纤维素含量较高。然而，象草表面的木质素液滴和芦苇秸秆中的高木质素含量限制了它们的水解性能。通过增加纤维素含量和去除木质素的有机溶剂和碱预处理后的芦苇秸秆获得了较高的水解产率。然而，大象草的水解仅通过有机溶剂预处理得到增强。进一步的研究表明，添加牛血清白蛋白 (BSA) 或带有 His- 和 S-Tags (Trx-His-S) 的硫氧还蛋白改善了碱预处理大象草的水解。特别是，Trx-His-S 首次被用作木质纤维素糖化的添加剂。它的结构和催化性能被认为有利于酶水解。结论象草和芦苇秸秆可作为生物转化的原料。有机溶剂和碱预处理提高了它们的酶促糖产量；然而，预处理的象草水解产量的增加不如芦苇秸秆有效。在碱预处理的象草水解过程中，Trx-His-S作为添加剂表现良好，其结构和催化能力有利于酶解。