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The metagenome-assembled genome of Candidatus Oenococcus aquikefiri from water kefir represents the species Oenococcus sicerae.
Food Microbiology ( IF 5.3 ) Pub Date : 2019-12-27 , DOI: 10.1016/j.fm.2019.103402
Marko Verce 1 , Luc De Vuyst 1 , Stefan Weckx 1
Affiliation  

Recently, a metagenomic study of a water kefir fermentation ecosystem enabled the reconstruction of a metagenome-assembled genome (MAG) of an Oenococcus species that was different from the three species of this genus known so far. Therefore, the name Candidatus Oenococcus aquikefiri was proposed for this novel Oenococcus species. In the meantime, however, a fourth member of the genus, Oenococcus sicerae, isolated from French cider, was reported. The comparison of its genome sequence with the Candidatus O. aquikefiri MAG showed an average nucleotide identity (ANI) value of 98.53%. In addition, the 16S rRNA and pheS genes of the two species were 99.4% and 99.9% identical, respectively. As the presence of O. sicerae in a water kefir metagenome was also revealed by metagenomic recruitment plotting, it can be stated that Candidatus O. aquikefiri and O. sicerae belong to the same species. Intraspecies variations include the presence or absence of a citrate lyase operon and components of various phosphotransferase (PTS) transport systems.

中文翻译:

由水牛乳中的念珠菌念珠菌组成的基因组组装基因组代表了干燥的Oenococcus sicerae。

最近,一项对水开菲尔发酵生态系统的宏基因组学研究使得能够重建与迄今为止已知的该属的三个属不同的Oenococcus属的一个由元基因组组装的基因组(MAG)。因此,对于这种新颖的Oenococcus种类,提出了名称Candidatus Oenococcus aquikefiri的名称。然而,与此同时,据报道从法国苹果酒中分离出的第四种干燥的Oenococcus sicerae。其基因组序列与C.idad。Oquikefiri MAG的比较表明,其平均核苷酸同一性(ANI)值为98.53%。此外,两个物种的16S rRNA和pheS基因分别具有99.4%和99.9%的同一性。由于宏基因组募集图还揭示了水牛乳气菌基因组中存在橙色拟南芥(O. sicerae),因此可以说是假丝酵母O. aquikefiri和O. 干燥小麦属于同一物种。种内变异包括是否存在柠檬酸裂合酶操纵子和各种磷酸转移酶(PTS)转运系统的组成部分。
更新日期:2019-12-27
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