Abstract Abdominal aortic aneurysm (AAA) is the vascular disease forming when aorta in the abdomen swells. AAA with the size of 5.5 and above has possibility to rupture and leads a death. Insulin-like Growth Factor 1 (IGF1) is the circulating biomarker for AAA diagnosis, in this study IGF1 was detected by the improved Enzyme linked Immunoassay (ELISA). The conventional ELISA necessitates the improvement due to the lesser sensitivity than modern sensors. Higher biomolecular immobilization is one of the wise ways to enhance the sensitivity. This study was aimed to capture a higher number of IGF1 on ELISA surface through the aminated silica (SiO2) nanoparticle with the size measured to be 80−100 nm. On the amine-SiO2 modified surface, Glutaraldehyde (GLU) complexed IGF1 was chemically bonded. It was found that 400 pM of IGF1 detected on amine-SiO2 modified ELISA, whereas the conventional ELISA without nanoparticle mediation shows the limit of detection as 12.5 nM with ∼30 times lower detection. The selectivity experiment was carried out with the mixture of IGF1 and relevant proteins (IGF2 and IGFBP3), and it was noticed that anti-IGF1 selectively detected IGF1. This nanoparticle-mediated surface immobilization captured a higher rate of antigen on a larger surface created for high-performance detection.

中文翻译：

---

在 ELISA 中使用胺化二氧化硅纳米颗粒检测胰岛素样生长因子 1 的改进免疫测定法

摘要 腹主动脉瘤（AAA）是腹部主动脉膨胀时形成的血管疾病。5.5及以上的AAA有可能破裂导致死亡。胰岛素样生长因子 1 (IGF1) 是用于 AAA 诊断的循环生物标志物，在本研究中，IGF1 是通过改进的酶联免疫测定 (ELISA) 检测到的。由于灵敏度低于现代传感器，传统 ELISA 需要改进。更高的生物分子固定化是提高灵敏度的明智方法之一。本研究旨在通过尺寸为 80-100 nm 的胺化二氧化硅 (SiO2) 纳米颗粒在 ELISA 表面捕获更多 IGF1。在胺-SiO2 改性的表面上，戊二醛 (GLU) 复合的 IGF1 化学键合。发现在胺-SiO2 修饰的 ELISA 上检测到 400 pM IGF1，而没有纳米颗粒介导的常规 ELISA 显示检测限为 12.5 nM，检测值低约 30 倍。用IGF1和相关蛋白（IGF2和IGFBP3）的混合物进行选择性实验，发现抗IGF1选择性检测IGF1。这种纳米颗粒介导的表面固定在为高性能检测而创建的更大表面上捕获了更高的抗原率。