To further elucidate the roles of T and B lymphocytes in fish subunit and DNA candidate vaccines for immunisation, the immune responses of T and B lymphocytes to recombinant protein (rOmpK) and plasmid OmpK (pOmpK) from Vibrio anguillarum plus cyclosporine A (CsA) were investigated in flounder (Paralichthys olivaceus). The results showed that in the rOmpK-immunised groups, the percentages of CD4-1+ and CD4-2+ T (PCD4-1+ and PCD4-2+ T) lymphocytes significantly increased to a peak on days 5 or 7. The percentages of IgM+ B (PIgM+ B) lymphocytes and specific antibodies markedly increased to a peak at weeks 4 or 5. The nine immune-related genes were significantly up-regulated and the expression levels of CD4-1, CD4-2 and MHC II genes were higher than that of CD8α, CD8β and MHC I genes. The CD4+ T lymphocytes, IgM+ B lymphocytes, and specific antibodies were significantly inhibited by CsA. Therefore, the responses of CD4+ T lymphocytes influenced the responses of the B lymphocytes and antibodies. In the pOmpK-immunised groups, the PCD4-1+, PCD4-2+, and PCD8β+ T lymphocytes significantly increased to a peak on days 11 or 14, days 9 or 11, and days 7 or 9, respectively. The PIgM+ B lymphocytes and specific antibodies significantly increased to a peak at weeks 5 or 6. Immune related genes upregulated, and CD4+ and CD8+ T lymphocytes, IgM+ B lymphocytes and specific antibodies all suppressed by CsA, suggesting that the responses of T lymphocytes subpopulations influenced B lymphocytes and antibodies responses. Therefore, the subpopulations of T lymphocytes played an important role in the immune responses induced by subunit and DNA candidate vaccines of OmpK and regulated the immune responses of B lymphocytes in flounder.

中文翻译：

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两种形式的鳗弧菌外膜蛋白K诱导比目鱼（Paralichthys olivaceus）的T和B淋巴细胞免疫反应：亚单位疫苗和DNA疫苗。

为了进一步阐明T和B淋巴细胞在鱼亚基和DNA候选疫苗中的免疫作用，分别对T和B淋巴细胞对鳗鳗弧菌和环孢菌素A（CsA）的重组蛋白（rOmpK）和质粒OmpK（pOmpK）的免疫应答。在比目鱼（Paralichthys olivaceus）中进行了调查。结果显示，在rOmpK免疫组中，CD4-1 +和CD4-2 + T（PCD4-1 +和PCD4-2 + T）淋巴细胞的百分比在第5天或第7天显着增加。 IgM + B（PIgM + B）淋巴细胞和特异性抗体的显着增加在第4或5周达到峰值。这9个免疫相关基因被显着上调，CD4-1，CD4-2和MHC II基因的表达水平达到了峰值。高于CD8α，CD8β和MHC I基因。CD4 + T淋巴细胞，IgM + B淋巴细胞，CsA可显着抑制特异性抗体。因此，CD4 + T淋巴细胞的反应影响B淋巴细胞和抗体的反应。在经pOmpK免疫的组中，PCD4-1 +，PCD4-2 +和PCD8β+ T淋巴细胞分别在第11或14天，第9或11天以及第7或9天显着增加至峰值。PIgM + B淋巴细胞和特异性抗体在第5或6周显着增加至峰值。免疫相关基因上调，CD4 +和CD8 + T淋巴细胞，IgM + B淋巴细胞和特异性抗体均被CsA抑制，表明T淋巴细胞亚群的反应受到影响B淋巴细胞和抗体反应。所以，