BACKGROUND In Guided Tissue Regeneration (GTR), barrier membranes are used to allow selective cell populations to multiply and to promote periodontal regeneration. A frequent complication is membrane exposure to the oral cavity followed by bacterial colonization. The purpose of this in-vitro-study was to elucidate, if rinsing with a chlorhexidine digluconate solution (CHX) prevents bacterial adhesion, and whether it interferes with attachment of periodontal ligament (PDL) fibroblasts and epithelial cells to membrane surfaces. METHODS Firstly, two bioresorbable membranes (polylactide-based and collagen-based) were dipped into 0.06% CHX and 0.12% CHX, before biofilms (2-species representing periodontal health, 6-species representing a periodontitis) were formed for 2 h and 8 h. Subsequently, colony forming units (cfu) were counted. Secondly, the membranes were treated with CHX and inoculated in bacteria suspension two-time per day for 3 d before cfu were determined. In additional series, the influence of CHX and bacterial lysates on attachment of epithelial cells and PDL fibroblasts was determined. Parameter-free tests were applied for statistical analysis. RESULTS Cfu in "healthy" biofilms did not differ between the two membranes, more cfu were counted in "periodontitis" biofilm on collagen than on polylactide membranes. One-time dipping of membranes into CHX solutions did not markedly influence the cfu counts of both biofilms on polylactide membrane; those on collagen-based membrane were significantly reduced with being 0.12% CHX more active than 0.06% CHX. More-fold CHX dipping of membranes reduced concentration-dependent the cfu counts of both biofilms on both membranes. In general, the number of attached gingival epithelial cells and PDL fibroblasts was higher on collagen than on polylactide membrane. Lysates of the periodontopathogenic bacteria inhibited attachment of PDL fibroblasts to membranes. CHX decreased in a concentration-dependend manner the number of attached gingival epithelial cells and PDL fibroblasts. CONCLUSIONS The present in-vitro results appear to indicate that membranes in GTR should only be used when bacteria being associated with periodontal disease have been eliminated. An exposure of the membrane should be avoided. Rinsing with CHX may prevent or at least retard bacterial colonization on membrane exposed to the oral activity. However, a certain negative effect on wound healing cannot be excluded.

中文翻译：

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二葡萄糖氯己定在基于聚丙交酯和基于胶原的膜上的体外抗生物膜活性。

背景技术在引导组织再生（GTR）中，屏障膜用于允许选择性细胞群繁殖并促进牙周再生。常见的并发症是膜暴露于口腔，随后细菌定植。这项体外研究的目的是阐明，如果用洗必太二葡萄糖酸溶液（CHX）冲洗可防止细菌粘附，以及它是否干扰牙周膜（PDL）成纤维细胞和上皮细胞附着于膜表面。方法首先，将两个生物可吸收膜（基于聚丙交酯和胶原蛋白的膜）浸入0.06％CHX和0.12％CHX中，然后形成生物膜（代表牙周健康的2种，代表牙周炎的6种）2 h和8 H。随后，计数菌落形成单位（cfu）。其次，膜用CHX处理并每天两次在细菌悬浮液中接种3天，然后测定cfu。在其他系列中，确定了CHX和细菌裂解物对上皮细胞和PDL成纤维细胞附着的影响。将无参数测试应用于统计分析。结果“健康”生物膜的Cfu在两张膜之间没有差异，胶原蛋白“牙周炎”生物膜中的cfu计数高于聚乳酸膜。一次性将膜浸入CHX溶液中并没有显着影响聚乳酸膜上两个生物膜的cfu计数。胶原蛋白膜上的那些显着降低，活性比0.06％CHX高0.12％CHX。CHX浸渍膜的倍数降低了两种膜上两种生物膜的浓度依赖性cfu计数。通常，在胶原蛋白上附着的牙龈上皮细胞和PDL成纤维细胞的数量要比在聚丙交酯膜上的数量高。牙周病原菌的裂解物抑制了PDL成纤维细胞附着在膜上。CHX以浓度依赖性方式减少附着的牙龈上皮细胞和PDL成纤维细胞的数量。结论目前的体外结果似乎表明，只有在消除了与牙周疾病有关的细菌后，才应使用GTR中的膜。应避免膜暴露。用CHX冲洗可能会阻止或至少延迟细菌暴露于口腔活动后在膜上的定植。然而，