In recent years, DNA has been regarded as a reliable raw material for building biological computers and biochips due to its nanoscale size, ultralow energy consumption, and high-performance computing potential. As the basis of building a biological computer, the research on the construction of nanoscale logic arithmetic and nanoscale biochemical logic circuits based on DNA molecules as carriers has attracted increasing attention. Although researchers use DNA strand replacement systems to achieve it this requires adjustment and careful design of the toehold, making sequence selection more difficult. To reduce dependence on the toehold, we propose a 3-way DNAzyme complex composed of three E6 DNAzymes assembled using the biological characteristics of E6 DNAzyme. This complex enriched the recognition vector of E6 DNAzyme, which can be used for multiple substrates, thus improving the reusability and efficiency of DNA molecules. At the same time, based on the 3-way DNAzyme complex and without the involvement of the toehold, we designed logic gates such as the OR gate, the AND gate, and the INHIBIT gate, and realized the construction of a new half subtractor and nanoscale biochemical logic circuit. These explorations and attempts extended the practicality of the 3-way DNAzyme complex. We believe that these logic elements will have a wide range of applications in DNA nanoscale programming, biological computing, and nanoscale medicine.

中文翻译：

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基于三向DNAzyme复合体的逻辑门设计

近年来，由于其纳米级的尺寸，超低的能耗和高性能的计算潜力，DNA被视为构建生物计算机和生物芯片的可靠原料。作为构建生物计算机的基础，以DNA分子为载体构建纳米级逻辑算术和纳米生化逻辑电路的研究日益受到关注。尽管研究人员使用DNA链替换系统来实现这一点，但这需要调整和仔细设计脚趾，使序列选择更加困难。为了减少对脚趾的依赖性，我们提出了一种三向DNAzyme复合物，该复合物由使用E6 DNAzyme的生物学特性组装而成的三个E6 DNAzyme组成。这种复合物丰富了E6 DNAzyme的识别载体，可以用于多种底物，从而提高了DNA分子的可重复使用性和效率。同时，基于三向DNAzyme复合体，并且没有脚趾的参与，我们设计了逻辑门（例如OR门，AND门和INHIBIT门），并实现了新的半减法器和纳米级生化逻辑电路。这些探索和尝试扩展了3-way DNAzyme复合物的实用性。我们相信这些逻辑元素将在DNA纳米级编程，生物计算和纳米级医学中有广泛的应用。和INHIBIT门，并实现了新的半减法器和纳米级生化逻辑电路的构建。这些探索和尝试扩展了3-way DNAzyme复合物的实用性。我们相信这些逻辑元素将在DNA纳米级编程，生物计算和纳米级医学中有广泛的应用。和INHIBIT门，并实现了新的半减法器和纳米级生化逻辑电路的构建。这些探索和尝试扩展了3-way DNAzyme复合物的实用性。我们相信这些逻辑元素将在DNA纳米级编程，生物计算和纳米级医学中有广泛的应用。