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P. falciparum gametocyte density and infectivity in peripheral blood and skin tissue of naturally infected parasite carriers in Burkina Faso.
The Journal of Infectious Diseases ( IF 6.4 ) Pub Date : 2019-12-26 , DOI: 10.1093/infdis/jiz680
Elamaran Meibalan 1, 2 , Aissata Barry 3, 4 , Matthew P Gibbins 5 , Shehu Awandu 4 , Lisette Meerstein-Kessel 4 , Fiona Achcar 5 , Selina Bopp 1 , Christopher Moxon 5 , Amidou Diarra 3 , Siaka Debe 3 , Nicolas Ouédraogo 3 , Ines Barry-Some 3 , Emilie S Badoum 3 , Traoré Fagnima 6 , Kjerstin Lanke 4 , Bronner P Gonçalves 7 , John Bradley 8 , Dyann Wirth 1 , Chris Drakeley 7 , Wamdaogo Moussa Guelbeogo 3 , Alfred B Tiono 3 , Matthias Marti 1, 5 , Teun Bousema 4, 7
Affiliation  

BACKGROUND Plasmodium falciparum transmission depends on mature gametocytes that can be ingested by mosquitoes taking a bloodmeal on human skin. Although gametocyte skin sequestration has long been hypothesized as important contributor to efficient malaria transmission, this has never been formally tested. METHODS In naturally infected gametocyte carriers from Burkina Faso, we assessed infectivity to mosquitoes by direct skin feeding and membrane feeding. We directly quantified male and female gametocytes and asexual parasites in: i) finger prick blood, ii) venous blood, iii) skin biopsies, and in pools of mosquitoes that fed iv) on venous blood or, v) directly on skin. Gametocytes were visualized in skin tissue by confocal microscopy. RESULTS Whilst more mosquitoes became infected when feeding directly on the skin compared to venous blood (odds ratio 2.01; 95% CI 1.21 - 3.33, p = 0.007), concentrations of gametocytes in the subdermal skin vasculature were not higher compared to other blood compartments; only sparse gametocytes were observed in skin tissue. DISCUSSION Our data strongly suggest that there is no significant skin sequestration of P. falciparum gametocytes. Gametocyte densities in peripheral blood are thus informative for predicting onward transmission potential to mosquitoes and can be used to target and monitor malaria elimination initiatives.

中文翻译:

布基纳法索自然感染寄生虫携带者外周血和皮肤组织中的恶性疟原虫配子体密度和传染性。

背景恶性疟原虫的传播依赖于成熟的配子体,这些配子体可以被吸食人体皮肤上的血粉的蚊子摄取。尽管长期以来人们一直认为配子体皮肤隔离是疟疾有效传播的重要因素,但这从未经过正式测试。方法 在来自布基纳法索的自然感染的配子体载体中,我们通过直接皮肤喂养和膜喂养来评估对蚊子的感染性。我们直接量化了雄性和雌性配子体和无性寄生虫:i) 手指刺血,ii) 静脉血,iii) 皮肤活检,以及在 iv) 静脉血或 v) 直接在皮肤上喂食的蚊子池中。通过共聚焦显微镜观察皮肤组织中的配子体。结果 虽然与静脉血相比,直接以皮肤为食的蚊子更多(优势比 2.01;95% CI 1.21 - 3.33,p = 0.007),但与其他血液隔室相比,皮下皮肤脉管系统中的配子体浓度并不高;在皮肤组织中仅观察到稀疏的配子体。讨论 我们的数据强烈表明恶性疟原虫配子体没有明显的皮肤隔离。因此,外周血中的配子体密度可用于预测蚊子的向前传播潜力,并可用于瞄准和监测消除疟疾的举措。讨论 我们的数据强烈表明恶性疟原虫配子体没有明显的皮肤隔离。因此,外周血中的配子体密度可用于预测蚊子的向前传播潜力,并可用于瞄准和监测消除疟疾的举措。讨论 我们的数据强烈表明恶性疟原虫配子体没有明显的皮肤隔离。因此,外周血中的配子体密度可用于预测蚊子的向前传播潜力,并可用于瞄准和监测消除疟疾的举措。
更新日期:2019-12-27
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