In the fabrication of cardiac tissue, an important factor is continuous measurement of its contraction features. A module that allows for a dynamic system capable of noninvasive and label-free monitoring of the contraction profile under administering chemicals and drugs is highly valuable for understanding accurate tissue mechanobiology. In this research, we have successfully demonstrated the use of surface plasmon resonance (SPR) technology for the first time to characterize the contractility of cardiac cells in response to Blebbistatin and ATP drug exposure in real tme. An optimal flow rate of 10 μL/min was selected for a continuous flow of warm media,and 10 μM drug administration effect was detected with high spatiotemporal sensitivity on contracting cardiomyocytes. Our drug screening has identified the source of the SPR periodic signal to be direct cell contraction rather than action potentials or calcium signaling. Per our results, SPR has high potential in applications in least-interference real-time and label-free tissue characterizations and cellular properties analysis from a functional and structural point of view.

中文翻译：

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基于非侵入性等离子的心脏药物对心肌细胞功能行为的实时表征。

在心脏组织的制造中，重要的因素是连续测量其收缩特征。允许动态系统能够在施用化学药品和药物的情况下无创且无标签地监测收缩曲线的模块，对于理解准确的组织力学生物学非常有价值。在这项研究中，我们已经成功地证明了表面等离子体共振（SPR）技术的首次使用来表征心脏在真实tme中对Blebbistatin和ATP药物反应后心脏细胞的收缩性。选择连续流动的温热介质的最佳流速为10μL/ min，并以对时空敏感性高的时空敏感性检测到10μM的药物对心肌细胞的收缩作用。我们的药物筛选已确定SPR周期信号的来源是直接细胞收缩，而不是动作电位或钙信号传导。根据我们的结果，从功能和结构的角度来看，SPR在最小干扰实时和无标签组织表征以及细胞特性分析中的应用具有很高的潜力。