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Four bottlenecks restrict colonization and invasion by the pathogen Ralstonia solanacearum in resistant tomato.
Journal of Experimental Botany ( IF 6.9 ) Pub Date : 2020-03-25 , DOI: 10.1093/jxb/erz562
Marc Planas-Marquès 1 , Jonathan P Kressin 2, 3 , Anurag Kashyap 1 , Dilip R Panthee 3 , Frank J Louws 2, 4 , Nuria S Coll 1 , Marc Valls 1, 5
Affiliation  

Ralstonia solanacearum is a bacterial vascular pathogen causing devastating bacterial wilt. In the field, resistance against this pathogen is quantitative and is available for breeders only in tomato and eggplant. To understand the basis of resistance to R. solanacearum in tomato, we investigated the spatio-temporal dynamics of bacterial colonization using non-invasive live monitoring techniques coupled to grafting of susceptible and resistant varieties. We found four 'bottlenecks' that limit the bacterium in resistant tomato: root colonization, vertical movement from roots to shoots, circular vascular bundle invasion, and radial apoplastic spread in the cortex. Radial invasion of cortical extracellular spaces occurred mostly at late disease stages but was observed throughout plant infection. This study shows that resistance is expressed in both root and shoot tissues, and highlights the importance of structural constraints to bacterial spread as a resistance mechanism. It also shows that R. solanacearum is not only a vascular pathogen but spreads out of the xylem, occupying the plant apoplast niche. Our work will help elucidate the complex genetic determinants of resistance, setting the foundations to decipher the molecular mechanisms that limit pathogen colonization, which may provide new precision tools to fight bacterial wilt in the field.

中文翻译:

四个瓶颈限制了病原菌青枯病菌在抗性番茄中的定殖和入侵。

Ralstonia solanacearum 是一种细菌性血管病原体,可导致破坏性青枯病。在田间,对这种病原体的抗性是定量的,并且仅在番茄和茄子中可供育种者使用。为了了解番茄中青枯菌抗性的基础,我们使用非侵入性实时监测技术结合易感和抗性品种的嫁接研究了细菌定植的时空动态。我们发现了限制抗性番茄中细菌的四个“瓶颈”:根定植、从根到芽的垂直运动、圆形维管束入侵和皮层中的径向质外体扩散。皮层细胞外空间的径向侵入主要发生在疾病晚期,但在整个植物感染过程中观察到。该研究表明,抗性在根和茎组织中都有表达,并强调了结构限制对细菌传播作为抗性机制的重要性。这也表明青枯菌不仅是维管病原体,而且从木质部扩散出来,占据植物质外体生态位。我们的工作将有助于阐明抗性的复杂遗传决定因素,为破译限制病原体定植的分子机制奠定基础,这可能为该领域对抗青枯病提供新的精确工具。
更新日期:2020-03-26
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