In this study, an attempt was made to investigate the mechanistic insight to identify the links between enzymatic and sonolysis processes through degradation mechanism. Experimental results revealed that ultrasound and cavitation increase the number of collision in enzymatic process through intense convection in the medium generated by transient cavitation, and enhances degradation efficiency by increasing interaction between enzyme and organic molecules. The formation of pH· radicals in Bisphenol-A degradation is significant and it needs external binding agent such as polyethylene glycol (PEG) to prevent the blocking of reactive sites of enzymes. On the other hand, Ciprofloxacin is susceptible to enzyme, and thus the degradation enhancement is not much significant in sono-enzymatic process. The highest degradation rates at optimum condition of pH 7.0 and 25 °C were 66.52% and 68.41% for Bisphenol-A and Ciprofloxacin, respectively. The antimicrobial activity test and the LCMS results revealed that the intermediates (acetic acid, ethyl alcohol and other products with –OH group) formed during sono-enzymatic reaction are less toxic as compared to that formed in enzymatic process. This essentially means that the enzymatic process initiates the degradation reaction and ultrasound helps towards complete mineralization by degrading the intermediates. To the best of our knowledge, this is the first report on detailed degradation mechanism of Bisphenol-A and Ciprofloxacin using enzymatic and sono-enzymatic processes.

在这项研究中，试图调查机械的见解，以通过降解机理来识别酶促过程和声分解过程之间的联系。实验结果表明，超声和空化通过瞬时空化在介质中产生的强对流而增加了酶过程中的碰撞次数，并通过增加酶与有机分子之间的相互作用来提高降解效率。pH自由基在双酚A降解中的形成非常重要，它需要外部结合剂（例如聚乙二醇（PEG））来防止酶的反应位点被阻断。另一方面，环丙沙星对酶敏感，因此降解的增强在声-酶过程中不是很明显。在最佳条件下，pH 7.0和25°C，双酚A和环丙沙星的最高降解率分别为66.52％和68.41％。抗菌活性测试和LCMS结果表明，与酶促过程相比，声纳酶促反应过程中形成的中间体（乙酸，乙醇和其他带有–OH基团的产物）的毒性较小。这实质上意味着酶促过程会引发降解反应，而超声波则通过降解中间体来帮助完成矿化作用。据我们所知，这是关于双酚A和环丙沙星使用酶促和声酶促降解的详细降解机理的第一份报告。抗菌活性测试和LCMS结果表明，与酶促过程相比，声纳酶促反应过程中形成的中间体（乙酸，乙醇和其他带有–OH基团的产物）的毒性较小。这实质上意味着酶促过程会引发降解反应，而超声波则通过降解中间体来帮助完成矿化作用。据我们所知，这是关于双酚A和环丙沙星使用酶促和声酶促降解的详细降解机理的第一份报告。抗菌活性测试和LCMS结果表明，与酶促过程相比，声纳酶促反应过程中形成的中间体（乙酸，乙醇和其他带有–OH基团的产物）的毒性较小。这实质上意味着酶促过程会引发降解反应，而超声波则通过降解中间体来帮助完成矿化作用。据我们所知，这是关于双酚A和环丙沙星使用酶促和声酶促降解的详细降解机理的第一份报告。这实质上意味着酶促过程会引发降解反应，而超声波则通过降解中间体来帮助完成矿化作用。据我们所知，这是关于双酚A和环丙沙星使用酶促和声酶促降解的详细降解机理的第一份报告。这实质上意味着酶促过程会引发降解反应，而超声波则通过降解中间体来帮助完成矿化作用。据我们所知，这是关于双酚A和环丙沙星使用酶促和声酶促降解的详细降解机理的第一份报告。