Abstract The fluorescence quenching rate ( K Q ) of fluorescence dye in continuous-wave stimulated emission depletion (CW-STED) system is experimentally investigated by utilizing a commercial dye (Coumarin 102). With the plotting of a power-dependence curve, it is found that the quenching rate is increasing rather than staying steady with the raising of depletion beam power in CW STED. Moreover, increasing fluorescence dye concentration and excitation power could also result in the increasing of K Q , which gives rise to the negative influence on spatial resolution of CW STED microscope. The results are helpful in understanding the influence of quenching on CW STED, improving the performance of CW STED microscope and developing new STED-compatible fluorescent dye.

中文翻译：

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增强的香豆素 102 在受激发射耗尽中的荧光猝灭率

摘要 利用市售染料(Coumarin 102) 实验研究了连续波受激发射耗尽(CW-STED) 系统中荧光染料的荧光猝灭率(KQ)。通过绘制功率依赖曲线，发现在 CW STED 中，随着耗尽束功率的增加，淬火速率增加而不是保持稳定。此外，增加荧光染料浓度和激发功率也会导致 KQ 增加，从而对 CW STED 显微镜的空间分辨率产生负面影响。研究结果有助于了解猝灭对 CW STED 的影响，提高 CW STED 显微镜的性能，开发新的 STED 兼容荧光染料。