Zika virus (ZIKV) is a mosquito-borne flavivirus (FLAV) that emerged in Brazil in 2015 and has rapidly spread to more than 50 countries worldwide. However, early, accurate, and specific point-of-care (POC) diagnosis of ZIKV is very difficult because most infected patients are asymptomatic or display nonspecific symptoms similar to those of other viral infections, and most of the analysis also requires instruments. Herein, an instrument-free ZIKV POC test using a drop of blood comprising a vial immunosensor, artificial nanozyme platinum/gold core-shell nanoparticles (Pt@Au NPs) as a signal probe, and a smartphone was developed to specifically detect ZIKV without cross-reaction with other FLAVs. A high sensitivity of 1 pg/mL ZIKV, desirable specificity, data storage, and geographic location surveillance were simultaneously achieved for the proposed POC test. Our POC test suitably met the urgent needs of ports of entry, airports, and endemic regions with stressed resources, as well as strict clinical requirements for ZIKV detection.

中文翻译：

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使用带有智能手机的无酶小瓶免疫传感器对Zika病毒进行检测和感染监测的血清学现场检查。

寨卡病毒（ZIKV）是一种由蚊子传播的黄病毒（FLAV），于2015年在巴西出现，并已迅速传播到全球50多个国家。但是，对ZIKV进行早期，准确且特定的即时护理（POC）诊断非常困难，因为大多数被感染的患者无症状或显示出与其他病毒感染相似的非特异性症状，并且大多数分析还需要仪器。在本文中，开发了一种使用一瓶血液的无仪器ZIKV POC测试，该测试包括一个小瓶免疫传感器，人工纳米酶铂/金核壳纳米粒子（Pt @ Au NPs）作为信号探针，以及智能手机，专门用于无交叉检测ZIKV与其他FLAV的反应。灵敏度高达1 pg / mL ZIKV，具有所需的特异性，数据存储，拟议的POC测试同时实现了地理位置监视和地理位置监视。我们的POC测试可以满足在资源紧张的情况下进入港口，机场和流行地区的紧急需求，以及ZIKV检测的严格临床要求。