Propolis has beneficial effects anti-inflammatory, anti-diabetes and anti-obesity in human or murine models,but its mechanism has not been fully elucidated. This study was to investigate the effects of ethanol extract of propolis (EEP) on the gut microbiota, and to analyze the associations between these alterations of gut microbiota and insulin resistance and obesity in high fat diet (HFD)-fed mice.Male C57BL/6J mice were fed with chow diet, high-fat diet, and high-fat diet supplemented with 1% EEP or 2%EEP. EEP supplementation reduced HFD-induced weight gain and liver fat accumulation, proinflammatory cytokines and insulin resistance, and improved glucose tolerance and lipid profile. Meanwhile, EEP supplementation in HFD-fed mice increased anti-obesity and anti-inflammatory bacteria such as genera Roseburia and Intestinimonas and species Parabacteroides goldsteinii and Parabacteroides distasonis, and reduced pro-inflammatory bacteria such as genera Faecalibaculum and Prevotella and species Bacteroides vulgatus. These dominant bacterial taxa altered by EEP were significantly associated with the metabolic parameters of insulin resistance and obesityin HFD-fed mice. The results in this study indicated that EEP reduced HFD-induced obesity and insulin resistant, which may be mediated by modulating the composition and function of gut microbiota.

蜂胶在人或鼠模型中具有抗炎，抗糖尿病和抗肥胖的有益作用，但其机理尚未完全阐明。这项研究旨在调查蜂胶乙醇提取物（EEP）对肠道菌群的影响，并分析这些肠道菌群变化与高脂饮食（HFD）喂养的小鼠的胰岛素抵抗和肥胖之间的关系。给6J小鼠喂以低脂饮食，高脂饮食和补充1％EEP或2％EEP的高脂饮食。补充EEP减少了HFD引起的体重增加和肝脂肪蓄积，促炎性细胞因子和胰岛素抵抗，并改善了糖耐量和血脂水平。同时，在HFD喂养的小鼠中补充EEP会增加抗肥胖和抗炎细菌，例如Roseburia属和Intestinimonas和物种Parabacteroides goldsteinii和Parabacteroides distasonis，并减少促炎细菌如属Faecalibaculum和普雷沃和物种杆菌普通拟。这些由EEP改变的优势细菌类群与HFD喂养的小鼠的胰岛素抵抗和肥胖症的代谢参数显着相关。这项研究的结果表明，EEP降低了HFD诱导的肥胖和胰岛素抵抗，这可能是通过调节肠道菌群的组成和功能来介导的。