Bone defects caused by cancer surgery or trauma have a strong negative impact on human health. Treatment with cell and material-based complexes provides an alternative strategy for the regeneration of damaged bone tissue. The good physical properties and suitable biodegradability of a thermosensitive hydrogel has been shown to act as a valuable scaffold. Platelet derived growth factor BB (PDGFBB) is mainly secreted by platelets and promotes the migration and angiogenesis of mesenchymal stem cells (MSCs). Although PDGFBB is known to indirectly enhance bone repair in vivo, the effects of PDGFBB on stem cells from apical papilla (SCAPs) require further investigation. In our study, the proliferation of cells was investigated by the cell counting kit-8 and live/dead staining methods. The results indicated that PDGFBB promoted the proliferation of SCAPs. Real-time polymerase chain reaction and Western blot experiments were used to detect osteogenic genes and proteins. Moreover, calvarial defects were created in Sprague-Dawley rats and different complexes implanted. The results shown by micro-CT and hematoxylin and eosin analysis demonstrated that the hydrogel combined with lentiviral supernatant-green fluorescent protein-PDGFBB significantly improved new bone formation and mineralization compared with the other three groups. In summary, our research showed that a thermosensitive hydrogel can be used as a scaffold for 3D cell culture, and PDGFBB gene-modified SCAPs can improve bone formation in calvarial defects.

中文翻译：

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来自顶端乳头和热敏性水凝胶支架的PDGFBB修饰的干细胞诱导了骨再生。

由癌症手术或外伤引起的骨骼缺陷对人体健康具有强烈的负面影响。用基于细胞和材料的复合物进行治疗为受损骨组织的再生提供了另一种策略。已经显示出热敏性水凝胶的良好物理性质和合适的生物降解性可作为有价值的支架。血小板衍生的生长因子BB（PDGFBB）主要由血小板分泌，并促进间充质干细胞（MSCs）的迁移和血管生成。尽管已知PDGFBB可以间接增强体内骨骼修复，但PDGFBB对根尖乳头（SCAPs）干细胞的作用尚需进一步研究。在我们的研究中，通过细胞计数试剂盒8和活/死染色方法研究了细胞的增殖。结果表明PDGFBB促进了SCAPs的增殖。实时聚合酶链反应和蛋白质印迹实验被用来检测成骨基因和蛋白质。此外，在Sprague-Dawley大鼠中植入了颅盖骨缺损，并植入了不同的复合物。显微CT，苏木精和曙红分析显示的结果表明，与其他三组相比，水凝胶结合慢病毒上清液-绿色荧光蛋白-PDGFBB可以显着改善新骨的形成和矿化。总而言之，我们的研究表明，热敏性水凝胶可以用作3D细胞培养的支架，而PDGFBB基因修饰的SCAPs可以改善颅骨缺损中的骨形成。在Sprague-Dawley大鼠中创建颅骨缺损，并植入了不同的复合物。显微CT，苏木精和曙红分析显示的结果表明，与其他三组相比，水凝胶结合慢病毒上清液-绿色荧光蛋白-PDGFBB可以显着改善新骨的形成和矿化。总而言之，我们的研究表明，热敏性水凝胶可以用作3D细胞培养的支架，而PDGFBB基因修饰的SCAPs可以改善颅骨缺损中的骨形成。在Sprague-Dawley大鼠中创建颅骨缺损，并植入了不同的复合物。显微CT，苏木精和曙红分析显示的结果表明，与其他三组相比，水凝胶结合慢病毒上清液-绿色荧光蛋白-PDGFBB可以显着改善新骨的形成和矿化。总而言之，我们的研究表明，热敏性水凝胶可以用作3D细胞培养的支架，而PDGFBB基因修饰的SCAPs可以改善颅骨缺损中的骨形成。