Many gram-positive bacteria produce bacillithiol to aid in the maintenance of redox homeostasis and degradation of toxic compounds, including the antibiotic fosfomycin. Bacillithiol is produced via a three-enzyme pathway that includes the action of the zinc-dependent deacetylase BshB. Previous studies identified conserved aspartate and histidine residues within the active site that are involved in metal binding and catalysis, but the enzymatic mechanism is not fully understood. Here we report two X-ray crystallographic structures of BshB from Bacillus subtilis that provide insight into the BshB catalytic mechanism.

中文翻译：

---

BshB的X射线晶体学结构，锌依赖性脱乙酰基酶参与了杆菌硫醇的生物合成。

许多革兰氏阳性细菌会产生乙硫醇，以帮助维持氧化还原稳态和降解有毒化合物，包括抗生素磷霉素。乙硫醇是通过包括锌依赖性脱乙酰基酶BshB的作用在内的三种酶途径产生的。先前的研究确定了活性位点中与金属结合和催化有关的保守的天冬氨酸和组氨酸残基，但是酶的机理尚不完全清楚。在这里，我们报告了枯草芽孢杆菌的BshB的两个X射线晶体学结构，这些结构为BshB催化机理提供了见识。