Chemically modified genes and genomes with customized properties will become a valuable tool in numerous fields, including synthetic biology, biotechnology, and medicine. These genetic materials are meant to store and exchange information with DNA and RNA while tuning their functionality. Herein, we outline the development of an alternative genetic system carrying phosphoramidate linkages that successfully propagates genetic information in bacteria and at the same time is labile to acidic conditions. The P3'→N5' phosphoramidate-containing DNA (PN-DNA) was enzymatically synthesized by using 5'-amino-2',5'-deoxycytidine 5'-N-triphosphates (NH-dCTPs) as substrates for DNA polymerases and employed to encode antibiotic resistance in Escherichia coli. The resulting PN-DNA can be efficiently destroyed by mild acidic conditions, whereas an unmodified counterpart remains intact. A cloning strategy was proposed for assembling modified fragments into a genome. This method can be of interest to scientists working in the field of orthogonal nucleic acid genes and genomes.

中文翻译：

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来自磷酸氨基磷酸酯-DNA的遗传信息的体内表达。

具有定制属性的化学修饰的基因和基因组将成为许多领域的宝贵工具，包括合成生物学，生物技术和医学。这些遗传材料旨在在调节其功能的同时与DNA和RNA进行存储和交换信息。在此，我们概述了携带氨基磷酸酯键的可替代遗传系统的开发，该系统成功地在细菌中传播了遗传信息，同时对酸性条件也很不稳定。以5'-氨基-2'，5'-脱氧胞苷5'-N-三磷酸酯（NH-dCTPs）为DNA聚合酶的底物酶促合成了含P3'→N5'氨基磷酸酯的DNA（PN-DNA）并使用编码大肠杆菌中的抗生素抗性。所产生的PN-DNA可以在弱酸性条件下被有效破坏，而未修改的副本保持完整。提出了将修饰的片段组装到基因组中的克隆策略。该方法可能对从事正交核酸基因和基因组研究的科学家感兴趣。