Growing attention has been paid to the importance of bound polyphenols in dietary fiber. This study aimed to elucidate the effect of bound polyphenols on the fermentation and antioxidant properties of carrot dietary fiber (CDF) in vivo and in vitro. Compared with CDF treatment, 16S rRNA pyrosequencing of in vivo mice feces and in vitro human fecal fermentation samples showed that dephenolized carrot dietary fiber (CDF-DF) treatment decreases operational taxonomic units (OTUs), ACE and Chao1 indexes, increases Firmicute/Bacteroidetes ratio and relative abundance (RA) of Parabacteroides at phylum, restrains RAs of typical beneficial bacteria as well as improves RAs of various harmful bacteria at genus. Meanwhile, short-chain fatty acid (SCFA) contents were lower, while the pH value was higher in the CDF-DF group than those in the CDF group. Interestingly, the combination of bound polyphenols and CDF-DF (CDDP) could recover these indexes influenced by the removal of bound polyphenols in in vitro fermentation samples. Furthermore, the CDF-DF-fed mice exhibited higher MDA content and lower SOD and GSH-Px activities in the colon. The cellular antioxidant activity (CAA) value of CDF-DF was lower than that of CDF and CDDP. These results revealed that bound polyphenols significantly contribute to the fermentation and antioxidant properties of CDF.

中文翻译：

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体内和体外结合多酚对胡萝卜膳食纤维发酵和抗氧化性能的影响。

膳食纤维中结合的多酚的重要性日益引起人们的关注。这项研究旨在阐明体内和体外结合的多酚对胡萝卜膳食纤维（CDF）发酵和抗氧化特性的影响。与CDF处理相比，体内小鼠粪便和体外人类粪便发酵样品的16S rRNA焦磷酸测序显示，脱酚胡萝卜膳食纤维（CDF-DF）处理降低了操作分类单位（OTU），ACE和Chao1指数，增加了Firmicute / Bacteroidetes比率菌门处的副细菌的相对丰度（RA）可以抑制典型有益细菌的RA并改善属下各种有害细菌的RA。同时，与CDF组相比，CDF-DF组的短链脂肪酸（SCFA）含量较低，而pH值较高。有趣的是，结合多酚和CDF-DF（CDDP）的组合可以恢复受体外发酵样品中结合多酚去除影响的这些指标。此外，由CDF-DF喂养的小鼠在结肠中显示出更高的MDA含量和更低的SOD和GSH-Px活性。CDF-DF的细胞抗氧化活性（CAA）值低于CDF和CDDP。这些结果表明，结合的多酚显着促进了CDF的发酵和抗氧化性能。CDF-DF的细胞抗氧化活性（CAA）值低于CDF和CDDP。这些结果表明，结合的多酚显着促进了CDF的发酵和抗氧化性能。CDF-DF的细胞抗氧化活性（CAA）值低于CDF和CDDP。这些结果表明，结合的多酚显着促进了CDF的发酵和抗氧化性能。