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Microbial community and antibiotic resistance profiles of biomass and effluent are distinctly affected by antibiotic addition to an anaerobic membrane bioreactor
Environmental Science: Water Research & Technology ( IF 5 ) Pub Date : 2019/12/23 , DOI: 10.1039/c9ew00913b
Ali Zarei-Baygi 1, 2, 3, 4 , Moustapha Harb 1, 2, 3, 4, 5 , Phillip Wang 1, 2, 3, 4 , Lauren B. Stadler 4, 5, 6, 7 , Adam L. Smith 1, 2, 3, 4
Affiliation  

The transfer of antibiotic resistance to pathogenic bacteria is one of the most eminent human health threats and a concern in water reuse schemes. Anaerobic membrane bioreactors (AnMBRs) are an emerging wastewater treatment biotechnology with significant potential for mainstream wastewater treatment. However, AnMBR effluents remain largely unexplored with respect to their microbial community composition and their antibiotic resistance profiles. In this study, we operated a bench-scale AnMBR for the treatment of domestic wastewater containing antibiotics (250 μg L−1 each of sulfamethoxazole, ampicillin, and erythromycin) and evaluated microbial community structure and antibiotic resistance gene (ARG) dynamics in both the biomass and effluent. Results showed that ARG abundances in the biomass of the AnMBR consistently increased throughout the experiment, while the effluent ARG abundances saw a sharp increase upon initial antibiotic exposure to the system and then dropped immediately thereafter. Further, a vastly more variable microbial community was observed in the AnMBR effluent as compared to the biomass. Several potentially pathogenic genera in the effluent were strongly correlated with the abundance of specific resistance genes (e.g., sul1), as well as a class 1 integrase gene (intI1). Overall, the results of this study provide useful insights into the association of ARGs with microbial community dynamics in AnMBR, which is needed to devise operational and design strategies to lessen dissemination of antibiotic resistance to the environment.

中文翻译:

厌氧膜生物反应器中添加抗生素会明显影响生物量和污水的微生物群落和抗生素耐药性

抗生素对病原菌的抗性转移是人类最严重的健康威胁之一,也是中水回用计划中的一个关注点。厌氧膜生物反应器(AnMBRs)是一种新兴的废水处理生物技术,具有主流废水处理的巨大潜力。然而,就其微生物群落组成和抗生素抗药性而言,仍未开发出AnMBR废水。在这项研究中,我们使用了台式AnMBR来处理含抗生素(250μgL -1分别使用磺胺甲恶唑,氨苄青霉素和红霉素),并评估了生物量和废水中的微生物群落结构和抗生素抗性基因(ARG)动态。结果表明,在整个实验过程中,AnMBR生物量中的ARG丰度持续增加,而出水的ARG丰度在最初将抗生素暴露于系统后急剧增加,然后立即下降。此外,与生物质相比,在AnMBR废水中观察到的微生物群落变化更大。在流出物中有几个潜在致病属同丰的特定抗性基因(显着相关例如sul1),以及一类1整合酶基因(intI1)。总体而言,这项研究的结果为ARGs与AnMBR中微生物群落动态的关联提供了有用的见识,这对于设计操作和设计策略以减少对环境的抗生素耐药性的传播是必要的。
更新日期:2020-03-05
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