Spontaneous Raman micro‐spectroscopy has been demonstrated great potential in delineating tumor margins; however, it is limited by slow acquisition speed. We describe a superpixel acquisition approach that can expedite acquisition between ~×100 and ×10 000, as compared to point‐by‐point scanning by trading off spatial resolution. We present the first demonstration of superpixel acquisition on rapid discrimination of basal cell carcinoma tumor from eight patients undergoing Mohs micrographic surgery. Results have been demonstrated high discriminant power for tumor vs normal skin based on the biochemical differences between nucleus, collagen, keratin and ceramide. We further perform raster‐scanned superpixel Raman imaging on positive and negative margin samples. Our results indicate superpixel acquisition can facilitate the use of Raman microspectroscopy as a rapid and specific tool for tumor margin assessment.

中文翻译：

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超像素拉曼光谱仪可快速评估皮肤癌边缘。

自发拉曼显微光谱技术已被证明在描绘肿瘤边缘方面具有巨大的潜力。但是，它受到缓慢的采集速度的限制。我们描述了一种超像素采集方法，与通过权衡空间分辨率进行逐点扫描相比，该方法可以在〜×100到×10000之间加速采集。我们目前正在对8位接受Mohs显微外科手术治疗的患者快速鉴别基底细胞癌肿瘤进行超像素采集的首次演示。根据细胞核，胶原蛋白，角蛋白和神经酰胺之间的生化差异，结果已证明对肿瘤和正常皮肤具有高判别力。我们进一步对正和负边距样本执行光栅扫描超像素拉曼成像。