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Virus-based SELEX (viro-SELEX) allows development of aptamers targeting knotty proteins.
Analyst ( IF 4.2 ) Pub Date : 2019-12-23 , DOI: 10.1039/c9an01943j
Chandan Narayan 1 , Junyoung Kwon , Chonsaeng Kim , Seong-Jun Kim , Sung Key Jang
Affiliation  

It has been 100 years since the worst flu (Spanish flu) mankind has ever experienced. Rapid, accurate diagnosis and subtyping of flu are still an urgent unmet medical need. By using surrogate virus-based SELEX (viro-SELEX), we report here multiple advances incorporated into the field of flu diagnostics: (i) aptamers that can bind to the native virus well even though they cannot bind strongly to a recombinant protein (hemagglutinin); (ii) a couple of aptamers that can target a broad range of strains belonging to the H1N1 subtype and detect only the H1N1 subtype and nothing else; (iii) a highly sensitive lateral flow assay system (limit of detection is 0.08 HAU) using fluorescence-tagged aptamers. The viro-SELEX method of aptamer selection in conjunction with a fluorescent tag on aptamers is a very useful approach to develop highly sensitive, specific, portable, rapid, and quantitative point-of-care testing diagnostic tools for the future.

中文翻译:

基于病毒的SELEX(viro-SELEX)允许开发针对结蛋白的适体。

自人类经历过最严重的流感(西班牙流感)以来已有100年了。快速,准确的流感诊断和分型仍然是迫切需要满足的医疗需求。通过使用基于替代病毒的SELEX(viro-SELEX),我们在此报告了流感诊断领域的多项进展:(i)即使不能牢固结合重组蛋白(血凝素),也可以很好地结合天然病毒的适体); (ii)一对适体,它们可以靶向属于H1N1亚型的多种菌株,仅检测H1N1亚型,而不能检测其他任何一种;(iii)使用荧光标记的适体的高度灵敏的侧向血流分析系统(检测极限为0.08 HAU)。适体选择的viro-SELEX方法与适体上的荧光标记结合使用,是开发高度敏感,
更新日期:2020-02-17
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