Azo dyes can metabolize back to precursor aromatic amines (arylamines), which are potentially carcinogenic. The ISO 14362-1:2017 standard requires the determination of the arylamines, produced from textile samples, preferably by using the gas chromatography mass spectrometric (GC-MS) method. This paper presents an ion-pairing high performance liquid chromatography tandem mass spectrometric (LC-MS/MS) method for determining aromatic amines, derived from azo colorants, in both natural and synthetic textiles. The separation enables adequate apparent retention factor (k’ > 2.1 for the most hydrophilic compounds) and has appropriate sensitivity without sample clean-up procedure. The background matrix constituents influence the quantification in even a 100-fold diluted sample, therefore, calibration requires background compensation. The method allows fast confirmation and quantification of twenty-five arylamines in complex matrices of textiles. The method was validated with success and used to both natural and synthetic textile proficiency test (PT) samples.

偶氮染料可以代谢回潜在的前体芳香胺（芳基胺），可能具有致癌性。ISO 14362-1：2017标准要求测定从纺织品样品中产生的芳胺，最好使用气相色谱质谱（GC-MS）方法。本文提出了一种离子对高效液相色谱串联质谱（LC-MS / MS）方法，用于测定天然和合成纺织品中源自偶氮染料的芳香胺。分离能够获得足够的表观保留因子（对于大多数亲水性化合物，k'> 2.1），并且具有适当的灵敏度，而无需样品清理程序。背景基质成分甚至会影响100倍稀释样品中的定量，因此，校准需要背景补偿。该方法可以快速确认和定量纺织品复杂基质中的25种芳基胺。该方法已成功验证，可用于天然和合成纺织品能力测试（PT）样品。