Due to the biological features of sesquiterpene coumarins and incomparable interest in therapeutics application of natural products, extraction of sesquiterpene coumarins from asafoetida have gained highly attention. One of the most important problems is removal of sulfur containing compounds which are co-existed with sesquiterpene coumarins. So employment of new methods for selective extraction and cleanup of sesquiterpene coumarins is very substantial. In this study using dummy molecularly imprinting technique, 7-hydroxycoumarin-imprited polymer was synthesized and after evaluation of binding properties of polymers, the optimum one was used as sorbent in solid phase extraction. Afterwards dummy molecularly imprinting solid phase extraction (DMISPE) method was calibrated for simultaneous extraction of galbanic acid, 7-isopentenyloxy coumarin and auraptene from aqueous media before high performance liquid chromatography with UV detector (HPLC-UV) analysis. The recovery was in the range of 68.32%-84.69%, which were in the acceptable range compared to previous works. Finally, the calibrated DMISPE method was used for extraction and cleanup of sesquiterpene coumarins from asafetida plant. The concentration of isosamarcandin, kellerin and farnesiferol in asafoetida extract was obtained 0.8, 2.7, and 5 µg/mL, respectively, using standard addition method.

由于倍半萜香豆素的生物学特性以及对天然产物的治疗应用的兴趣无与伦比，因此从asafoetida中提取倍半萜香豆素备受关注。最重要的问题之一是去除与倍半萜香豆素共存的含硫化合物。因此，采用新方法选择性提取和纯化倍半萜香豆素是非常重要的。在这项研究中，使用虚拟分子印迹技术合成了7-羟基香豆素印迹聚合物，并在评估了聚合物的结合性能后，将最佳的吸附剂用作固相萃取中的吸附剂。之后，对虚拟分子印迹固相萃取（DMISPE）方法进行了校正，以同时萃取出Galbanic酸，在使用高效液相色谱仪进行紫外检测器（HPLC-UV）分析之前，先从水性介质中提取7-异戊烯氧基香豆素和紫杉醇。回收率在68.32％-84.69％的范围内，与以前的工作相比处于可接受的范围内。最后，将校准的DMISPE方法用于从asafetida植物中提取和纯化倍半萜香豆素。使用标准添加方法分别获得了异叶苦杏仁甙提取物中异黄花can素，克勒林和法呢酚的浓度，分别为0.8、2.7和5 µg / mL。校准后的DMISPE方法用于提取和净化来自阿法夫蒂达植物的倍半萜香豆素。使用标准添加方法分别获得了异叶苦杏仁甙提取物中异黄花can素，克勒林和法呢酚的浓度，分别为0.8、2.7和5 µg / mL。校准后的DMISPE方法用于提取和净化来自阿法夫蒂达植物的倍半萜香豆素。使用标准添加方法分别获得了异叶苦杏仁甙提取物中异黄花can素，克勒林和法呢酚的浓度，分别为0.8、2.7和5 µg / mL。