当前位置: X-MOL 学术ACS Biomater. Sci. Eng. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Role of Stiffness versus Wettability in Regulating Cell Behaviors on Polymeric Surfaces
ACS Biomaterials Science & Engineering ( IF 5.8 ) Pub Date : 2020-01-06 , DOI: 10.1021/acsbiomaterials.9b01430
Fei Zan 1, 2 , Qiang Wei 2 , Liming Fang 1, 3 , Mengyue Xian 1, 2 , Yu Ke 4 , Gang Wu 1, 2
Affiliation  

Substrate wettability and stiffness, two factors impacting cell behaviors simultaneously, have been attracting much attention to elaborate which one dominates. In this study, hydrophilic poly(2-hydroxyethyl methacrylate) brushes were grafted onto the surfaces of poly(dimethylsiloxane) (PDMS) with elastic moduli of 3.66, 101.65 and 214.97 MPa and decreasing water contact angle from 120.4° to 38.5°. Cell behaviors of three cell lines including mBMSCs, ATDC-5, and C28/I2 were then investigated on the hydrophilic and hydrophobic PDMS with different stiffness, respectively. The proliferation of three cell lines was faster on the hydrophilic PDMS than the hydrophobic PDMS, but the stiffness of the hydrophilic or hydrophobic PDMS did not have a significant impact on cell proliferation. The increase of the stiffness enhanced cell migration, the cell spread and the gene expression proportion of extracellular matrix/intercellular adhesion molecules (integrin + FAK/NCAM + N-cadherin) for all three cell lines, but the increase of the wettability showed small enhancement in cell migration, cell spread and gene expression. Moreover, the cartilage-specific gene expression of SOX9 and COL2 downregulated for all three cell lines with the increasing stiffness. The interpretation of the effect of substrate wettability and stiffness on cell behaviors would function as very useful guideline to direct scaffold fabrication.

中文翻译:

刚度与可湿性在调节聚合物表面细胞行为方面的作用

基质润湿性和刚度是同时影响细胞行为的两个因素,因此引起人们的极大关注是要详细说明哪个是主导。在这项研究中,将亲水性聚甲基丙烯酸2-羟乙酯刷接在聚二甲基硅氧烷(PDMS)表面,其弹性模量为3.66、101.65和214.97 MPa,并且水接触角从120.4°减小到38.5°。然后分别在具有不同刚度的亲水性和疏水性PDMS上研究了包括mBMSCs,ATDC-5和C28 / I2在内的三种细胞系的细胞行为。在亲水性PDMS上,三种细胞系的增殖比疏水性PDMS快,但是亲水性或疏水性PDMS的刚度对细胞增殖没有显着影响。刚度的增加增强了细胞的迁移,三种细胞系的细胞扩散和细胞外基质/细胞间粘附分子(整合素+ FAK / NCAM + N-钙黏着蛋白)的基因表达比例,但润湿性的增加显示细胞迁移,细胞扩散和基因表达的增强很小。此外,随着硬度的增加,所有三个细胞系的SOX9和COL2软骨特异性基因表达均下调。基底润湿性和刚度对细胞行为的影响的解释将作为指导脚手架制造的非常有用的指南。随着硬度的增加,所有三种细胞系的SOX9和COL2软骨特异性基因表达均下调。基底润湿性和刚度对细胞行为的影响的解释将作为指导脚手架制造的非常有用的指南。随着硬度的增加,所有三种细胞系的SOX9和COL2软骨特异性基因表达均下调。基底润湿性和刚度对细胞行为的影响的解释将作为指导脚手架制造的非常有用的指南。
更新日期:2020-01-06
down
wechat
bug