Arrayed CRISPR-based screens emerge as a powerful alternative to pooled screens making it possible to investigate a wide range of cellular phenotypes that are typically not amenable to pooled screens. Here, we describe a solid-phase transfection platform that enables CRISPR-based genetic screens in arrayed format with flexible readouts. We demonstrate efficient gene knockout upon delivery of guide RNAs and Cas9/guide RNA ribonucleoprotein complexes into untransformed and cancer cell lines. In addition, we provide evidence that our platform can be easily adapted to high-throughput screens and we use this approach to study oncogene addiction in tumor cells. Finally demonstrating that the human primary cells can also be edited using this method, we pave the way for rapid testing of potential targeted therapies.

中文翻译：

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用于阵列 CRISPR 筛选的固相转染平台。

基于 CRISPR 的阵列筛选成为混合筛选的强大替代方案，使得研究通常不适合混合筛选的各种细胞表型成为可能。在这里，我们描述了一种固相转染平台，该平台能够以阵列格式进行基于 CRISPR 的遗传筛选，并具有灵活的读数。我们展示了将引导 RNA 和 Cas9/引导 RNA 核糖核蛋白复合物递送至未转化细胞系和癌细胞系中时的有效基因敲除。此外，我们提供的证据表明我们的平台可以轻松适应高通量筛选，并且我们使用这种方法来研究肿瘤细胞中的癌基因成瘾。最后证明人类原代细胞也可以使用这种方法进行编辑，我们为快速测试潜在的靶向疗法铺平了道路。