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Gene expression profiles in bovine granulocytes reflect the aberration of liver functions.
Animal Science Journal ( IF 2 ) Pub Date : 2019-12-20 , DOI: 10.1111/asj.13324
Keiichiro Kizaki 1, 2 , Tomomi Kageyama 1 , Noriyuki Toji 1, 2 , Katsuo Koshi 1 , Kouya Sasaki 1, 2 , Norio Yamagishi 1, 2 , Toshina Ishiguro-Oonuma 1, 2 , Toru Takahashi 1, 2 , Kazuyoshi Hashizume 1
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Liver performs several important functions; however, predicting its functions is difficult. Methods of analyzing gene expression profiles, for example, microarray, provide functional information of tissues. Liver and peripheral blood leukocytes (PBLs) were collected from Holstein cows subjected to two different physiological conditions (non-pregnant and pregnant), and PBLs were fractionated by gradient cell separation. RNA from PBLs and liver were applied to oligo-DNA microarray and reverse transcription-quantitative polymerase chain reaction (RT-qPCR). It revealed a group of stable bovine liver genes under constant physiological conditions. When they applied to physiological conditions including non-pregnant and pregnant, the profiles of some genes in liver were consistent with those in PBLs. Microarray data subjected to a principal component analysis (PCA) showed that the hepatic gene expression profiles were more consistent with those of granulocytes than mononuclear cells. The relationship of gene profiles in liver with granulocytes was confirmed by RT-qPCR and hierarchical cluster analysis. Gene profiles of granulocytes were more reliable than those of mononuclear cells, which reflected liver functions. These results suggest that the genes expressed in PBLs, particularly granulocytes, may be convenient bioindicators for the diagnosis of clinical disorder and/or detecting aberration of liver functions in cows subjected to different physiological conditions.

中文翻译:

牛粒细胞中的基因表达谱反映了肝功能的异常。

肝脏执行多项重要功能;然而,预测它的功能是困难的。分析基因表达谱的方法,例如微阵列,提供了组织的功能信息。从经历两种不同生理条件(未怀孕和怀孕)的荷斯坦奶牛中收集肝脏和外周血白细胞 (PBL),并通过梯度细胞分离对 PBL 进行分级分离。来自 PBL 和肝脏的 RNA 被应用于寡聚 DNA 微阵列和逆转录定量聚合酶链反应 (RT-qPCR)。它揭示了一组在恒定生理条件下稳定的牛肝基因。当它们应用于包括非妊娠和妊娠在内的生理条件时,肝脏中某些基因的谱与 PBL 中的一致。进行主成分分析 (PCA) 的微阵列数据显示,肝脏基因表达谱与粒细胞的基因表达谱比单核细胞更一致。通过 RT-qPCR 和层次聚类分析证实了肝脏中基因图谱与粒细胞的关系。粒细胞的基因谱比反映肝功能的单核细胞的基因谱更可靠。这些结果表明,在 PBL 中表达的基因,特别是粒细胞,可能是诊断临床疾病和/或检测处于不同生理条件下的奶牛肝功能异常的便捷生物指示剂。通过 RT-qPCR 和层次聚类分析证实了肝脏中基因图谱与粒细胞的关系。粒细胞的基因谱比反映肝功能的单核细胞的基因谱更可靠。这些结果表明,在 PBL 中表达的基因,特别是粒细胞,可能是诊断临床疾病和/或检测处于不同生理条件下的奶牛肝功能异常的便捷生物指示剂。通过 RT-qPCR 和层次聚类分析证实了肝脏中基因图谱与粒细胞的关系。粒细胞的基因谱比反映肝功能的单核细胞的基因谱更可靠。这些结果表明,在 PBL 中表达的基因,特别是粒细胞,可能是诊断临床疾病和/或检测处于不同生理条件下的奶牛肝功能异常的便捷生物指示剂。
更新日期:2019-12-20
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