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Comparative analysis of time-based and quadrat sampling in seasonal population dynamics of intermediate hosts of human schistosomes.
PLOS Neglected Tropical Diseases ( IF 3.8 ) Pub Date : 2019-12-20 , DOI: 10.1371/journal.pntd.0007938
Javier Perez-Saez 1 , Théophile Mande 1 , Dramane Zongo 2 , Andrea Rinaldo 1, 3
Affiliation  

BACKGROUND Despite their importance for designing and evaluating schistosomiasis control trials, little attention in the literature has been dedicated to sampling protocols for the parasite's snail intermediate hosts since their first development. We propose a comparative analysis of time-based and quadrat sampling protocols to quantify the seasonal variations in the abundance of these aquatic snail species of medical importance. METHODOLOGY/PRINCIPAL FINDINGS Snail populations were monitored during 42 consecutive months in three types of habitats (ephemeral pond, ephemeral river and permanent stream) in two sites covering different climatic zones in Burkina Faso. We employed both a widely used time-based protocol of 30min of systematic collection at a weekly interval, and a quadrat protocol of 8 replicates per sample at a monthly interval. The correspondence between the two protocols was evaluated using an ensemble of statistical models including linear and saturating-type functional forms as well as allowing for count zero-inflation. The quadrat protocol yielded on average a relative standard error of 40%, for a mean snail density of 16.7 snails/m2 and index of dispersion of 1.51. Both protocols yielded similar seasonal patterns in snail abundance, confirming the asynchrony between permanent and ephemeral habitats with respect to the country's seasonal rainfall patterns. Formal model comparison of the link between time vs. quadrat counts showed strong support of saturation for the latter and measurement zero-inflation, providing important evidence for the presence of density feedbacks in the snail's population dynamics, as well as for spatial clustering. CONCLUSIONS/SIGNIFICANCE In addition to the agreement with the time-based method, quadrat sampling provided insight into snail population dynamics and comparable density estimates across sites. The re-evaluation of these "traditional" sampling protocols, as well as the correspondence between their outputs, is of practical importance for the design and evaluation of schistosomiasis control trials.

中文翻译:

基于时间和二次抽样的人类血吸虫中间宿主季节性种群动态的比较分析。

背景技术尽管它们在设计和评估血吸虫病控制试验中很重要,但是自从它们的第一个发展以来,很少有文献关注寄生虫的蜗牛中间宿主的采样方案。我们建议对基于时间的采样方法和正交采样协议进行比较分析,以量化这些具有医学重要性的水生蜗牛物种的丰富度的季节性变化。方法/主要发现在覆盖布基纳法索不同气候区的两个地点的三种生境(短暂池塘,短暂河流和永久河流)中连续42个月监测了蜗牛种群。我们采用了一种广泛使用的基于时间的方案,即每周间隔30分钟进行系统收集,还采用了一种方形方案,每个样品每月进行8次重复。使用统计模型(包括线性和饱和类型的功能形式以及允许计数为零的通货膨胀)的整体统计模型对两种协议之间的对应关系进行了评估。正交方案平均产生的相对标准误差为40%,平均蜗牛密度为16.7蜗牛/平方米,分散指数为1.51。两种协议在蜗牛数量上都产生了相似的季节性模式,这证实了该国季节性降雨模式在永久性栖息地和短暂栖息地之间是不同步的。时间与二次方计数之间的联系的形式化模型比较显示,后者对饱和度和测量零通货膨胀的有力支持,为蜗牛种群动态中存在密度反馈以及空间聚类提供了重要证据。结论/意义除了与基于时间的方法达成一致之外,正交抽样还提供了对蜗牛种群动态的了解以及跨站点可比密度估计的见解。对这些“传统”采样方案及其输出之间的对应关系的重新评估对于血吸虫病对照试验的设计和评估具有实际意义。
更新日期:2019-12-21
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