当前位置: X-MOL 学术J. Mol. Recognit. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Recognition of gluconeogenic enzymes; Icl1, Fbp1, and Mdh2 by Gid4 ligase: A molecular docking study.
Journal of Molecular Recognition ( IF 2.7 ) Pub Date : 2019-12-20 , DOI: 10.1002/jmr.2831
Abdo A Elfiky 1, 2 , Alaa M Ismail 1 , Wael M Elshemey 1, 3
Affiliation  

The pro/N-degron pathway is an evolved protein degradation pathway through the ubiquitin-proteasome system. It is a vital pathway to attain protein homeostasis inside the liver cells with varying glucose levels. N-terminal proline exists in more than 300 proteins in Saccharomyces cerevisiae, but only three of them are the gluconeogenic enzymes; isocitrate lyase (Icl1), fructose-1,6-bisphosphatase (Fbp1), and malate dehydrogenase (Mdh2). The present in silico study aims to structurally illustrate the binding of Icl1 enzyme to Gid4 ligase concerning its peers; Fbp1 and Mdh2. Based on the molecular docking scores and interactions, one can attribute the binding stability of Gid4 with degrons, to peptides of length six up to eight from the N-terminal. Moreover, the percent change in the docking score provides a rationale for the unique Gid4-Icl11-4 interaction. The present study provides insights on the binding attitude of Gid4 ligase to degrons of different lengths, so one will consider in designing peptidomimetics to target Gid4 ligase.

中文翻译:

糖异生酶的识别;Gid4 连接酶的 Icl1、Fbp1 和 Mdh2:分子对接研究。

pro/N-degron 途径是通过泛素-蛋白酶体系统进化的蛋白质降解途径。这是在具有不同葡萄糖水平的肝细胞内实现蛋白质稳态的重要途径。N-末端脯氨酸存在于酿酒酵母中的300多种蛋白质中,但其中只有三种是糖异生酶;异柠檬酸裂解酶 (Icl1)、果糖-1,6-双磷酸酶 (Fbp1) 和苹果酸脱氢酶 (Mdh2)。目前的计算机研究旨在从结构上说明 Icl1 酶与 Gid4 连接酶的结合;Fbp1 和 Mdh2。根据分子对接评分和相互作用,可以将 Gid4 与 degron 的结合稳定性归因于从 N 端开始的长度为 6 到 8 的肽。而且,对接分数的百分比变化为独特的 Gid4-Icl11-4 相互作用提供了基本原理。本研究提供了关于 Gid4 连接酶与不同长度的 degron 的结合态度的见解,因此在设计靶向 Gid4 连接酶的拟肽时将考虑。
更新日期:2020-04-03
down
wechat
bug