RNA secondary structures play a key role in splicing, gene expression, microRNA biogenesis, RNA editing, and other biological processes. The importance of RNA structures has been demonstrated in the life cycle of RNA-containing viruses, including the influenza virus. At least two regions of conserved secondary structure in NS segment (+) RNA are predicted to vary among influenza virus strains with respect to thermodynamic stability; both fall in the NS1 open reading frame. The NS1 protein is involved in multiple virus-host interaction processes, and its main function is to inhibit the cellular immune response to viral infection. Using a reverse genetics approach, four influenza virus strains were constructed featuring mutations that have different effects on RNA secondary structure. Growth curve experiments and ELISA data show that, at least in the first viral replication cycle, mutations G123A and A132G affecting RNA structure in the (82-148) NS RNA region influence NS1 protein expression.

中文翻译：

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RNA二级结构的变化会在早期流感病毒感染期间影响NS1蛋白的表达。

RNA二级结构在剪接，基因表达，microRNA生物发生，RNA编辑和其他生物学过程中起关键作用。RNA结构的重要性已在包含RNA的病毒（包括流感病毒）的生命周期中得到证明。据预测，流感病毒株之间在热力学稳定性方面，NS区段（+）RNA中至少两个保守二级结构区域会有所不同。两者都属于NS1开放阅读框。NS1蛋白参与多种病毒-宿主相互作用过程，其主要功能是抑制细胞对病毒感染的免疫反应。使用反向遗传学方法，构建了四种流感病毒株，这些菌株的特征在于对RNA二级结构具有不同影响的突变。生长曲线实验和ELISA数据表明，