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Parasitological, serological and molecular survey of camel trypanosomiasis in Somalia.
Parasites & Vectors ( IF 3.2 ) Pub Date : 2019-12-21 , DOI: 10.1186/s13071-019-3853-5
Ahmed A Hassan-Kadle 1, 2 , Abdalla M Ibrahim 1 , Hamisi S Nyingilili 3 , Abdulkarim A Yusuf 1, 2 , Thállitha S W J Vieira 2 , Rafael F C Vieira 2, 4
Affiliation  

BACKGROUND Camel trypanosomiasis or surra is of great concern in Somalia, since the country possesses the largest one-humped camel (Camelus dromedarius) population in the world. Civil war in Somalia has resulted in the destruction of educational, research, economic and social structures, making the country scores very low for most humanitarian indicators. Previous studies on detection of Trypanosoma species in Somali camels have only been performed during the 1990s using standard trypanosome detection methods (STDM). Considering the lack of state-of-the-art knowledge on camel trypanosomiasis in Somalia, the present study aimed to assess the prevalence of Trypanosoma spp. in three districts of Somalia. METHODS A total of 182 blood samples from C. dromedarius from nomadic and dairy farms were evaluated using STDM, serological (CATT/T. evansi) and molecular (ITS1-PCR) methods. RESULTS All samples were negative for Trypanosoma spp. by STDM. A total of 125/182 (68.7%, 95% CI: 61.4-75.3%) camels were seropositive for T. evansi by CATT/T. evansi. Camels reared in nomadic system were more likely to be seropositive for T. evansi than those under dairy production system (OR: 5.6, 95% CI: 2.1-15.2, P = 0.0001). Five out of 182 (2.7%, 95% CI: 0.9-6.3%) camels tested positive for Trypanosoma sp. by ITS1-PCR. Sequencing of the ITS1 region of the Trypanosoma species detected herein revealed that camels were infected with T. evansi and T. simiae. CONCLUSIONS Trypanosoma evansi is highly prevalent in camels from the Banadir region of Somalia, particularly in nomadic herds. To our knowledge, this is the first study to confirm infections with T. evansi and T. simiae in Somali camels through DNA sequencing. Our data highlight the need for implementation of adequate control measures aiming to reduce the impact on camel production in the country.

中文翻译:

索马里骆驼锥虫病的寄生虫学,血清学和分子学调查。

背景技术骆驼锥虫病或古兰经在索马里备受关注,因为该国拥有世界上最大的单驼驼(Camelus dromedarius)人口。索马里的内战导致教育,研究,经济和社会结构的破坏,使该国在大多数人道主义指标上的得分非常低。以前关于检测索马里骆驼中锥虫物种的研究仅在1990年代使用标准锥虫检测方法(STDM)进行。考虑到在索马里缺乏关于骆驼锥虫病的最新知识,本研究旨在评估锥虫病的患病率。在索马里的三个地区。方法使用STDM,血清学(CATT / T。evansi)和分子(ITS1-PCR)方法。结果所有样本锥虫属均为阴性。由STDM。通过CATT / T,总共有125/182(68.7%,95%CI:61.4-75.3%)骆驼对伊凡丝锥虫呈血清反应阳性。埃文西 在游牧系统中饲养的骆驼比在乳品生产系统中饲养的骆驼更容易感染埃文斯锥虫(OR:5.6,95%CI:2.1-15.2,P = 0.0001)。182个骆驼中有5个(2.7%,95%CI:0.9-6.3%)的锥虫检测为阳性。通过ITS1-PCR。本文检测到的锥虫物种的ITS1区域的测序表明,骆驼感染了伊万氏锥虫和西米氏锥虫。结论埃文锥虫在索马里巴纳迪尔地区的骆驼中高度流行,特别是在游牧民族中。据我们所知,这是第一个证实埃文氏锥虫和T. T.感染的研究。通过DNA测序在索马里骆驼中发现simiae。我们的数据突出表明,有必要采取适当的控制措施,以减少对该国骆驼生产的影响。
更新日期:2019-12-21
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