Cancer metastasis is a Gordian knot for tumor diagnosis and therapy. Many studies have demonstrated that metastatic processes are inevitably affected by the tumor microenvironment. Histopathology is used universally as the gold standard for cancer diagnosis despite the lengthy preparation process and invasiveness.

Methods: Here, we introduced a supercontinuum and super-wide-tuning integrated multimodal platform, which combines the confocal, nonlinear and fluorescence lifetime microscopy with autoregulations, for label-free evaluation of fresh tissue and pathological sections. Based on various automated tunable lasers, synchronized and self-adjusting components and eight fast switching detection channels, the system features fast, large-field and subcellular-scale imaging of exogenous and endogenous fluorophores, nonlinear coherent scattering and lifetime contrast.

Results: With such an integrated multi-dimensional system, we searched the metastatic region by two-photon and three-photon excited autofluorescence, analyzed the cancer invasion by second harmonic generation and revealed the affected cellular metabolism by phasor-lifetime. We demonstrated the flexible measurement of multiple nonlinear modalities at NIR I and II excitation with a pre-compensation for group delay dispersion of ~7,000 fs² and low power of <40 mW, and of dual autofluorescence lifetime decays for phasor approach to decompose cancer-associated and disassociated components. This significantly revealed the metastatic and metabolic optical signatures of the whole colony of pancreatic cancers.

Conclusion: The synergistic effect of the system demonstrates the great potential to translate this technique into routine clinical applications, particularly for large-scale and quantitative studies of metastatic colonization.

癌症转移是肿瘤诊断和治疗的重要结节。许多研究表明，转移过程不可避免地受到肿瘤微环境的影响。尽管准备过程冗长且具有侵入性，但组织病理学仍被普遍用作癌症诊断的金标准。

方法：在这里，我们介绍了一个超连续谱和超宽广度的集成多峰平台，该平台将共聚焦，非线性和荧光寿命显微镜与自动调节相结合，可对新鲜组织和病理切片进行无标签评估。该系统基于各种自动可调激光器，同步和自调整组件以及八个快速切换检测通道，具有外源和内源荧光团的快速，大视野和亚细胞成像，非线性相干散射和寿命对比。

结果：利用这样一个集成的多维系统，我们通过两光子和三光子激发的自发荧光搜索了转移区域，通过二次谐波的产生分析了癌症的侵袭，并通过相量寿命揭示了受影响的细胞代谢。我们展示了NIR I和II激发下多种非线性模态的灵活测量方法，并预先补偿了约7,000 fs ^2的群时延弥散和<40 mW的低功率，以及相量法分解癌症的双自发荧光寿命衰减。相关和分离的组件。这显着揭示了胰腺癌整个菌落的转移性和代谢性光学特征。

结论：该系统的协同作用证明了将该技术转化为常规临床应用的巨大潜力，特别是对于转移性定植的大规模和定量研究。