Loading hydrogels with bioactive agents is an important method for expanding the functional application of hydrogels. However, how to improve the local administration and slow release of drugs from a hydrogel is a challenge when using hydrogels loaded with drugs. In this paper, we first developed adhesive liposomes (A-LIP) loaded with BMP-2. Then, we incorporated the A-LIP into PEG hydrogels based on the coordinated cross-linking principle of SH-PEG and Ag⁺, fabricating an injectable, antibacterial and self-healing multifunctional drug delivery system. The adhesive lipo-hydrogel (A-LIP-PEG) fabricated by mixing PEG hydrogels and adhesive liposomes can be locally injected into an osteoporotic fracture and bone marrow cavity, where A-LIP-PEG can release adhesive liposomes that adhere to the bone injury area and promote bone reconstruction. Based on the principle of electrostatic attraction, tissue nonspecific A-LIP were fabricated by grafting octadecylamine onto liposomes. Because of the coordination and cross-linking of thiolated polyethylene (SH-PEG) and Ag⁺, the A-LIP-PEG showed excellent injectability and self-healing properties; further, because of the presence of Ag⁺, the A-LIP-PEG showed effective inhibition of S. aureus and Escherichia coli. The liposomes released by the A-LIP-PEG were able to adhere to tissue. In vitro studies showed that A-LIP-PEG significantly promoted osteogenic differentiation and had no significant effect on cell proliferation. Compared with common lipo-hydrogel (LIP-PEG), the A-LIP-PEG had better tissue adhesion in vivo, which led to better osteogenic differentiation and faster local bone remodeling of osteoporotic fractures in rats. This research developed a novel hydrogel system with adhesive liposomes to expand the application of hydrogels.

用生物活性剂加载水凝胶是扩大水凝胶功能应用的重要方法。然而，当使用载有药物的水凝胶时，如何改善局部给药和从水凝胶中缓慢释放药物是一个挑战。在本文中，我们首先开发了载有BMP-2的粘性脂质体（A-LIP）。然后，我们根据SH-PEG和Ag ⁺的配位交联原理将A-LIP掺入PEG水凝胶中，制造出可注射，抗菌和自我修复的多功能药物递送系统。通过将PEG水凝胶和粘性脂质体混合制成的粘性脂质水凝胶（A-LIP-PEG）可以局部注入骨质疏松性骨折和骨髓腔中，其中A-LIP-PEG可以释放粘附到骨损伤区域的粘性脂质体并促进骨骼重建。基于静电吸引原理，通过将十八烷基胺接枝到脂质体上来制备组织非特异性A-LIP。由于硫醇化聚乙烯（SH-PEG）和Ag ⁺的配位和交联，A-LIP-PEG表现出优异的可注射性和自愈特性；此外，由于存在Ag ⁺，A-LIP-PEG对Aβ-LIP-PEG表现出有效的抑制作用。金黄色葡萄球菌和大肠杆菌。由A-LIP-PEG释放的脂质体能够粘附到组织上。体外研究表明，A-LIP-PEG显着促进成骨分化，并且对细胞增殖没有显着影响。与普通的脂质水凝胶（LIP-PEG）相比，A-LIP-PEG在体内具有更好的组织粘附性，从而导致大鼠骨质疏松性骨折具有更好的成骨分化和更快的局部骨重塑。这项研究开发了一种新型的带有粘性脂质体的水凝胶系统，以扩展水凝胶的应用。